{"id":"W2771920161","doi":"10.1111/pbi.12870","title":"Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration","year":2017,"lang":"en","type":"article","venue":"Plant Biotechnology Journal","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":335,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Genetics; Agricultural Biotechnology Research Center, Academia Sinica; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences; Chinese Academy of Sciences; Academia Sinica","keywords":"Biology; CRISPR; Mutagenesis; Protoplast; Cas9; Mutant; Genetics; Nicotiana tabacum; DNA; Insertional mutagenesis; Gene; Phytoene desaturase; Transfection; Molecular biology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007076309430220888,"score_gpt":0.2609948396821039,"score_spread":0.253918530251883,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}