{"id":"W2781815848","doi":"10.1016/j.stemcr.2017.12.004","title":"Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection","year":2018,"lang":"en","type":"article","venue":"Stem Cell Reports","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":71,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute of Child Health and Human Development; Division of Chemical, Bioengineering, Environmental, and Transport Systems; National Human Genome Research Institute; National Eye Institute; National Science Foundation of Sri Lanka; Research to Prevent Blindness; VitreoRetinal Surgery Foundation; National Institute of General Medical Sciences; John Merck Fund; Waisman Center; Brain and Behavior Research Foundation; University of Wisconsin-Madison; Brain Research Foundation; Burroughs Wellcome Fund; Foundation Fighting Blindness; National Institutes of Health; National Science Foundation","keywords":"Biology; Induced pluripotent stem cell; CRISPR; Genome editing; Cas9; Puromycin; Genome; Computational biology; Gene; Genetics; Cell sorting; Embryonic stem cell; Cell; Protein biosynthesis","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002261606,0.0001595831,0.0001746613,0.00005100959,0.00008587434,0.00001319224,0.00007821373,0.0001155949,0.00001908371],"category_scores_gemma":[9.456218e-7,0.0001652312,0.0001067323,0.00008958838,0.00004713712,0.000002759139,0.00004569751,0.00006601165,0.000002784889],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002016512,"about_ca_system_score_gemma":0.0000314052,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002276642,"about_ca_topic_score_gemma":0.00002523431,"domain_scores_codex":[0.9987233,0.00002504957,0.0004378962,0.0003847547,0.0001773886,0.0002516171],"domain_scores_gemma":[0.9992613,0.00000271007,0.0001957493,0.0003174837,0.0001383986,0.000084355],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000006805753,0.00003599542,0.0003813673,0.00008197725,0.00002305629,0.00001775491,0.0001760653,0.000726127,0.996877,9.298583e-7,0.0001237662,0.001549206],"study_design_scores_gemma":[0.0001387415,0.000329374,0.0009710694,0.00001822251,0.00002884047,0.00009244929,0.0001320422,0.00009807141,0.9919788,0.000003482405,0.006045765,0.0001631818],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9750249,0.0004511015,0.02175144,0.000005049323,0.0005545796,0.0002153147,0.000003787212,0.00002034825,0.001973435],"genre_scores_gemma":[0.9980713,0.00001830531,0.0002252777,0.00001023006,0.0005427673,0.00001062179,0.00001856415,0.00003106712,0.001071932],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02304629,"threshold_uncertainty_score":0.6737934,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006425518078481189,"score_gpt":0.2408215437813149,"score_spread":0.2343960257028337,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}