{"id":"W2781815848","doi":"10.1016/j.stemcr.2017.12.004","title":"Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection","year":2018,"lang":"en","type":"article","venue":"Stem Cell Reports","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":71,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute of Child Health and Human Development; Division of Chemical, Bioengineering, Environmental, and Transport Systems; National Human Genome Research Institute; National Eye Institute; National Science Foundation of Sri Lanka; Research to Prevent Blindness; VitreoRetinal Surgery Foundation; National Institute of General Medical Sciences; John Merck Fund; Waisman Center; Brain and Behavior Research Foundation; University of Wisconsin-Madison; Brain Research Foundation; Burroughs Wellcome Fund; Foundation Fighting Blindness; National Institutes of Health; National Science Foundation","keywords":"Biology; Induced pluripotent stem cell; CRISPR; Genome editing; Cas9; Puromycin; Genome; Computational biology; Gene; Genetics; Cell sorting; Embryonic stem cell; Cell; Protein biosynthesis","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003434111,0.0003074343,0.0002816493,0.0002396734,0.0001862572,0.0003671763,0.0004588287,0.0002893465,0.001125083],"category_scores_gemma":[0.0002714624,0.0001614131,0.0002417603,0.0002102719,0.0002626467,0.000154918,0.00039434,0.0005352517,0.0005515915],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001445347,"about_ca_system_score_gemma":0.0002273631,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003350066,"about_ca_topic_score_gemma":0.0007797119,"domain_scores_codex":[0.9996189,0.000053343,0.00005152343,0.00008742476,0.0001565642,0.00003221641],"domain_scores_gemma":[0.9997868,0.00005659487,0.00004782771,0.00005776022,0.0000297086,0.00002144513],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001129825,0.000007113064,0.000119393,0.00001866391,0.000003324203,0.0000561719,0.00001252065,0.0001233315,0.9971234,0.00009696448,0.00004689907,0.002380874],"study_design_scores_gemma":[0.000003618505,0.00004196413,0.000649697,0.00000226423,0.000006803346,0.0002947806,0.000005822707,0.0009342676,0.9958716,0.00003223561,0.002153267,0.000003778468],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7183822,0.001180051,0.2723151,0.0001592286,0.00008882356,0.0004691584,0.001846757,0.001299888,0.004258799],"genre_scores_gemma":[0.8823029,0.001222845,0.1072225,0.000122728,0.00002031579,0.0002971879,0.002657252,0.0002130142,0.005941251],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001125083,"threshold_uncertainty_score":0.003763855,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006425518078481189,"score_gpt":0.2408215437813149,"score_spread":0.2343960257028337,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}