{"id":"W2782353569","doi":"10.21769/bioprotoc.2674","title":"Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9","year":2018,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"","keywords":"CRISPR; Biology; Genome editing; Lytic cycle; Plasmid; Cas9; Genome; Homologous recombination; Virulence; Genetics; Bacteriophage; Lactococcus lactis; Gene; Cre-Lox recombination; Gene targeting; Temperateness; Computational biology; Phagemid; Bacteria; Virus; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0001247304,0.0001351717,0.0001362456,0.00003659039,0.0001805814,0.00003302595,0.0001939009,0.00006407169,0.01450401],"category_scores_gemma":[0.00001922224,0.0001202419,0.00007400528,0.000165303,0.0002367981,0.0002356017,0.0002092361,0.00008647324,0.0005961601],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001205116,"about_ca_system_score_gemma":0.00001131509,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000617639,"about_ca_topic_score_gemma":0.00006190472,"domain_scores_codex":[0.9990292,0.00003236959,0.0002946922,0.0002530353,0.0001306484,0.0002601167],"domain_scores_gemma":[0.9994711,0.000009000739,0.0001892624,0.0002338848,0.00002337796,0.00007338032],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003001914,0.0000830784,0.0006028674,0.00001891018,0.000008177884,0.000002394772,0.0001336298,0.00001033951,0.9963225,0.000004168726,0.001138591,0.001645332],"study_design_scores_gemma":[0.0005742059,0.0002098786,0.03671879,0.00004189391,0.00001317067,0.0000161094,0.00008316949,0.0003187522,0.6514515,0.00002120871,0.3103457,0.0002056121],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"empirical","genre_scores_codex":[0.4696248,0.000002106369,0.001832213,0.0001329441,0.0005595288,0.5143167,0.00008251402,0.0001177919,0.01333143],"genre_scores_gemma":[0.6124908,2.840925e-7,0.009413851,0.0003077214,0.001046243,0.3757288,0.000007117872,0.00004819853,0.0009570307],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.344871,"threshold_uncertainty_score":0.9863968,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02649473446568976,"score_gpt":0.3272003385640268,"score_spread":0.300705604098337,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}