{"id":"W2786398328","doi":"10.1242/dev.160333","title":"Genome and epigenome engineering CRISPR toolkit for <i>in vivo</i> modulation of <i>cis</i> -regulatory interactions and gene expression in the chicken embryo","year":2018,"lang":"en","type":"article","venue":"Development","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":89,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Medical Research Council Canada; Lister Institute of Preventive Medicine; Ovarian Cancer Action","keywords":"Biology; Epigenome; Enhancer; CRISPR; Cas9; Genome editing; Chromatin; Computational biology; Genetics; Genome engineering; Regulation of gene expression; Morpholino; Gene; Genome; Epigenetics; Cell biology; Gene expression; DNA methylation; Gene knockdown","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001749387,0.0000855658,0.00008416856,0.00005793253,0.00003315036,0.000009003143,0.0000589763,0.00004331712,0.000002444811],"category_scores_gemma":[0.00001482809,0.00007498357,0.00001363655,0.00004888832,0.00001912012,0.000004657825,0.00004949154,0.00003469521,1.889196e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001174471,"about_ca_system_score_gemma":0.0000191008,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003356749,"about_ca_topic_score_gemma":0.0000382302,"domain_scores_codex":[0.9994735,0.000009023302,0.0001797542,0.0001654572,0.00005280846,0.0001194302],"domain_scores_gemma":[0.9998005,0.00001337492,0.00002769782,0.0001121433,0.00002344342,0.00002283368],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001813142,0.00001394123,0.001041857,0.00002800405,0.000006597064,2.429183e-7,0.0008452945,0.0006749677,0.9965913,0.000007614487,0.0000173341,0.0007546778],"study_design_scores_gemma":[0.0003216736,0.00004569968,0.2124909,0.00002471239,0.000002987176,0.000004840816,0.00009411346,0.0005011825,0.7751324,0.00001037214,0.01126243,0.0001086908],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9612705,0.0004979781,0.03788332,0.00002724827,0.00006430022,0.0002064978,0.000005210767,0.000003071141,0.00004184006],"genre_scores_gemma":[0.9896556,0.00006000842,0.01006731,0.0000346236,0.00006716863,0.00004809836,0.00001950373,0.000009839529,0.00003787982],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.221459,"threshold_uncertainty_score":0.3057742,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007257499084222797,"score_gpt":0.2558542202136699,"score_spread":0.2485967211294471,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}