{"id":"W2786398328","doi":"10.1242/dev.160333","title":"Genome and epigenome engineering CRISPR toolkit for <i>in vivo</i> modulation of <i>cis</i> -regulatory interactions and gene expression in the chicken embryo","year":2018,"lang":"en","type":"article","venue":"Development","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":89,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Medical Research Council Canada; Lister Institute of Preventive Medicine; Ovarian Cancer Action","keywords":"Biology; Epigenome; Enhancer; CRISPR; Cas9; Genome editing; Chromatin; Computational biology; Genetics; Genome engineering; Regulation of gene expression; Morpholino; Gene; Genome; Epigenetics; Cell biology; Gene expression; DNA methylation; Gene knockdown","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000247406,0.0004086323,0.0002940872,0.0003691648,0.0002381806,0.0003983898,0.0004780099,0.0003577496,0.00178636],"category_scores_gemma":[0.0001594974,0.0003642323,0.0003250885,0.0001438069,0.0003398448,0.0002079261,0.0006437779,0.0006774079,0.0009380521],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003850253,"about_ca_system_score_gemma":0.000349702,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001462774,"about_ca_topic_score_gemma":0.003146941,"domain_scores_codex":[0.9997228,0.00003778738,0.00002749853,0.00007332201,0.00009638933,0.0000421383],"domain_scores_gemma":[0.9998636,0.00001960962,0.00004251717,0.00003316272,0.00001721777,0.00002391502],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001560411,0.000004619425,0.00004996464,0.00002429513,0.000002681101,0.00002841922,0.00001154795,0.0001535073,0.9981535,0.0002471094,0.0000569661,0.001251712],"study_design_scores_gemma":[0.000007155488,0.00005340939,0.00122466,0.00000786067,0.00001176166,0.0002361473,0.00001195161,0.001806761,0.989884,0.00006230634,0.006683743,0.00001003198],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7250664,0.00124061,0.255648,0.0002545285,0.0001324573,0.0005035463,0.003436851,0.004208222,0.009509372],"genre_scores_gemma":[0.8324062,0.0008621912,0.1478671,0.0001459455,0.00001365151,0.0004050863,0.002776549,0.0007594508,0.01476375],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00178636,"threshold_uncertainty_score":0.005975962,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007257499084222797,"score_gpt":0.2558542202136699,"score_spread":0.2485967211294471,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}