{"id":"W2788024007","doi":"10.1186/s13068-018-1031-1","title":"Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation","year":2018,"lang":"en","type":"article","venue":"Biotechnology for Biofuels","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; China Scholarship Council","keywords":"Pichia pastoris; 2,3-Butanediol; Plasmid; Fermentation; Bacillus subtilis; Escherichia coli; Heterologous; Klebsiella oxytoca; Biology; Strain (injury); Enantiomeric excess; Saccharomyces cerevisiae; Klebsiella pneumoniae; Chemistry; Gene; Biochemistry; Bacteria; Microbiology; Recombinant DNA; Genetics; Enantioselective synthesis; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003814162,0.0008356407,0.0008623514,0.000392331,0.0002587585,0.0005969222,0.0005009448,0.0004439534,0.0005479037],"category_scores_gemma":[0.0002341883,0.0003555433,0.0004400998,0.001044863,0.0001975966,0.0003822312,0.0003862447,0.0007097718,0.0007885461],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000481739,"about_ca_system_score_gemma":0.000318676,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001131931,"about_ca_topic_score_gemma":0.001351266,"domain_scores_codex":[0.9996973,0.00003331162,0.00005209738,0.00007157375,0.00009701296,0.00004877221],"domain_scores_gemma":[0.9998856,0.0000180748,0.00002570554,0.00001783972,0.00002346385,0.00002923423],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005913166,0.00005889343,0.0002082475,0.0002135599,0.00001189263,0.0001906992,0.0000339606,0.0004565059,0.9961625,0.0001149756,0.0001172534,0.002372329],"study_design_scores_gemma":[0.00002387821,0.0001758203,0.001725148,0.00002176621,0.00004212872,0.0002672429,0.00003953998,0.001747845,0.9887717,0.00007722852,0.00708505,0.00002258601],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.940491,0.00429751,0.03805725,0.0003652035,0.0001965285,0.0005418105,0.01017634,0.001066613,0.004807679],"genre_scores_gemma":[0.9060405,0.004385347,0.0644165,0.0001085002,0.00003554892,0.0003866829,0.01717274,0.0002550801,0.007199131],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001131931,"threshold_uncertainty_score":0.003495276,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008434256162359204,"score_gpt":0.2304369742918328,"score_spread":0.2220027181294736,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}