{"id":"W2788024007","doi":"10.1186/s13068-018-1031-1","title":"Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation","year":2018,"lang":"en","type":"article","venue":"Biotechnology for Biofuels","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; China Scholarship Council","keywords":"Pichia pastoris; 2,3-Butanediol; Plasmid; Fermentation; Bacillus subtilis; Escherichia coli; Heterologous; Klebsiella oxytoca; Biology; Strain (injury); Enantiomeric excess; Saccharomyces cerevisiae; Klebsiella pneumoniae; Chemistry; Gene; Biochemistry; Bacteria; Microbiology; Recombinant DNA; Genetics; Enantioselective synthesis; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000132104,0.0001285491,0.000138748,0.0001307981,0.00008036147,0.000007710805,0.00006280572,0.0003261788,0.000003015026],"category_scores_gemma":[0.0001094711,0.0001312278,0.00003388352,0.0001616454,0.0002458024,0.000009631534,0.00002654568,0.00005216764,5.930058e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000013449,"about_ca_system_score_gemma":0.00002571601,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004212697,"about_ca_topic_score_gemma":0.000001646128,"domain_scores_codex":[0.9992388,0.00001464008,0.0002203026,0.0003282268,0.00004572245,0.0001522386],"domain_scores_gemma":[0.999512,0.000001066406,0.0001321575,0.0001999182,0.000130803,0.00002404032],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005400322,0.00002027428,0.0001569845,0.00005301214,0.00003833758,4.261224e-8,0.00002858511,0.000005714927,0.9785551,0.0002709848,0.00001798838,0.02079896],"study_design_scores_gemma":[0.0002715979,0.0003249029,0.001185425,0.00001706628,0.00003364683,0.00006248357,0.00005999659,0.00009894551,0.9904129,0.00007517063,0.007323968,0.0001339259],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9118688,0.0001323061,0.08615758,0.0002509226,0.001114137,0.000333262,0.00009466911,0.00004687306,0.000001503247],"genre_scores_gemma":[0.9852409,0.0001908576,0.01355737,0.00001435054,0.0006177435,0.00001471114,0.0002638184,0.00001782517,0.00008239086],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07337219,"threshold_uncertainty_score":0.5351313,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008434256162359204,"score_gpt":0.2304369742918328,"score_spread":0.2220027181294736,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}