{"id":"W2790739701","doi":"10.1101/252163","title":"Selection-free, high frequency genome editing by homologous recombination of human pluripotent stem cells using Cas9 RNP and AAV6","year":2018,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Danmarks Frie Forskningsfond; Deutsche Forschungsgemeinschaft; Children's Health Research Institute; Sundhed og Sygdom, Det Frie Forskningsråd; Amon G. Carter Foundation","keywords":"Genome editing; Induced pluripotent stem cell; Cas9; CRISPR; Biology; Computational biology; Homologous recombination; Genome; Genetics; Gene; Embryonic stem cell","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000409853,0.0002628576,0.0001930084,0.0002382823,0.000133273,0.0003905763,0.0002603095,0.000305958,0.001151048],"category_scores_gemma":[0.0002228451,0.0001655536,0.0002382992,0.0001304678,0.0002303059,0.0001643308,0.0003583512,0.0005299809,0.0006461965],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001648754,"about_ca_system_score_gemma":0.0002064091,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003893467,"about_ca_topic_score_gemma":0.0003929735,"domain_scores_codex":[0.9996139,0.00006308778,0.00004172944,0.00008197856,0.0001685731,0.00003067089],"domain_scores_gemma":[0.9998673,0.0000282329,0.00003412993,0.00003557589,0.00001775895,0.00001704175],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001429877,0.000008702567,0.0001206198,0.00001618261,0.000003885516,0.00003771623,0.000009278547,0.0001358093,0.9969502,0.0002339967,0.00007138061,0.00239791],"study_design_scores_gemma":[0.00000484975,0.00003097936,0.0004528561,0.000002253107,0.000005075127,0.0002105536,0.000003967782,0.001276971,0.9949567,0.00006760978,0.002985386,0.000002818698],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8026454,0.0009391591,0.1880612,0.0002254017,0.0001097094,0.0001741863,0.0009363086,0.001329073,0.005579547],"genre_scores_gemma":[0.9319863,0.0004791433,0.05846883,0.00007058278,0.00001923915,0.00007959286,0.001032716,0.0001636891,0.0076999],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001151048,"threshold_uncertainty_score":0.003850639,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008075649312681515,"score_gpt":0.230540646114227,"score_spread":0.2224649968015455,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}