{"id":"W2791272017","doi":"10.1002/cbic.201800019","title":"Substrate Binding Drives Active‐Site Closing of Human Blood Group B Galactosyltransferase as Revealed by Hot‐Spot Labeling and NMR Spectroscopy Experiments","year":2018,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Blood groups and transfusion","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Universität Regensburg; Universität zu Lübeck; Deutsche Forschungsgemeinschaft; Victoria University; University of Victoria","keywords":"Chemistry; Substrate (aquarium); Acceptor; Allosteric regulation; Stereochemistry; Binding site; Nuclear magnetic resonance spectroscopy; Crystallography; Conformational change; Enzyme; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001523203,0.0002963863,0.0004745749,0.0001116177,0.0002213416,0.0000341587,0.00009151595,0.0001789592,0.000155032],"category_scores_gemma":[0.0000157725,0.0002857562,0.0001022353,0.0002267116,0.0002627447,0.0001397225,0.00003032462,0.0002536848,0.00001047338],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003071222,"about_ca_system_score_gemma":0.00002988058,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001136527,"about_ca_topic_score_gemma":0.000008800819,"domain_scores_codex":[0.9984153,0.00002733184,0.0003720926,0.0005177882,0.000271073,0.0003964395],"domain_scores_gemma":[0.9992507,0.00003098552,0.0001087238,0.000299739,0.00008788772,0.0002219451],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003640823,0.0005321992,0.01414855,0.0001985907,0.0001723923,0.0000198256,0.001799941,8.416586e-9,0.9824986,0.00006542014,0.00004666661,0.0001537116],"study_design_scores_gemma":[0.004605454,0.001092419,0.006027625,0.0005649892,0.0003414896,0.00004244637,0.0003517748,0.000005962346,0.986646,0.00003667937,0.00002927473,0.0002558861],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9966223,0.0009086962,0.00003796651,0.00007831002,0.00006417588,0.0003725369,0.00002665157,0.00007045695,0.001818911],"genre_scores_gemma":[0.998254,0.0002708729,0.0008493771,0.00007883919,0.0001936896,0.00001125742,0.0001209149,0.00005402735,0.0001670001],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008120922,"threshold_uncertainty_score":0.9999595,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.012632589869065,"score_gpt":0.2812570406788924,"score_spread":0.2686244508098274,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}