{"id":"W2791272017","doi":"10.1002/cbic.201800019","title":"Substrate Binding Drives Active‐Site Closing of Human Blood Group B Galactosyltransferase as Revealed by Hot‐Spot Labeling and NMR Spectroscopy Experiments","year":2018,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Blood groups and transfusion","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Universität Regensburg; Universität zu Lübeck; Deutsche Forschungsgemeinschaft; Victoria University; University of Victoria","keywords":"Chemistry; Substrate (aquarium); Acceptor; Allosteric regulation; Stereochemistry; Binding site; Nuclear magnetic resonance spectroscopy; Crystallography; Conformational change; Enzyme; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001715002,0.0001236896,0.0001517207,0.00005656219,0.0001104917,0.0002347333,0.0001558903,0.0001972869,0.0009705547],"category_scores_gemma":[0.0003090488,0.0001402492,0.00009544104,0.00007407228,0.0002225831,0.000171836,0.0001105602,0.0003329996,0.0001673118],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002897829,"about_ca_system_score_gemma":0.0001286542,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006697806,"about_ca_topic_score_gemma":0.0006094751,"domain_scores_codex":[0.9998991,0.0000199499,0.000004381818,0.00002425425,0.0000331091,0.00001909628],"domain_scores_gemma":[0.9998524,0.00006794065,0.000024657,0.00001447119,0.00001074742,0.00002977295],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008821458,0.00001318206,0.0001578486,0.00001235343,0.000001615418,0.00002676397,0.00002109754,0.00009484354,0.9987838,0.0001251956,0.00002177648,0.0006532326],"study_design_scores_gemma":[0.00001262895,0.00009613963,0.00261497,0.000001673189,0.000002457754,0.00006670928,0.00001627476,0.003564396,0.9931942,0.00007781186,0.0003481968,0.000004398124],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9977834,0.0001268237,0.001547469,0.00003760346,0.000006443755,0.000005144547,0.00003836718,0.00003434292,0.000420472],"genre_scores_gemma":[0.9986157,0.00005859019,0.0008399035,0.00002873205,0.000003497941,0.000005365436,0.00007173947,0.00001306655,0.0003633922],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009705547,"threshold_uncertainty_score":0.003246784,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.012632589869065,"score_gpt":0.2812570406788924,"score_spread":0.2686244508098274,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}