{"id":"W2795660791","doi":"10.1371/journal.pone.0195558","title":"Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells","year":2018,"lang":"en","type":"article","venue":"PLoS ONE","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":80,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hôpital Maisonneuve-Rosemont; Université Laval; Feldan (Canada)","funders":"National Research Council Canada","keywords":"CRISPR; Ribonucleoprotein; Trans-activating crRNA; Biology; Transfection; Recombinant DNA; Peptide; Cell biology; Gene delivery; Cas9; HEK 293 cells; Transcription (linguistics); Cell; Computational biology; Molecular biology; Gene; RNA; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002080564,0.0003998943,0.000293858,0.0001603797,0.0001591067,0.0003937249,0.0002381794,0.0005292429,0.000885049],"category_scores_gemma":[0.0001731866,0.0001825451,0.0002493052,0.0001112131,0.0003004576,0.0003853536,0.0004541393,0.000646454,0.0009827806],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002256094,"about_ca_system_score_gemma":0.0001665991,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001707427,"about_ca_topic_score_gemma":0.0001978906,"domain_scores_codex":[0.9998173,0.00002600814,0.0000152154,0.00004510992,0.00006332211,0.00003303461],"domain_scores_gemma":[0.9998679,0.00002904577,0.00005161856,0.00001591225,0.00001167542,0.00002383657],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000017706,0.000005821928,0.0000332759,0.00002751339,0.000002235459,0.00005115409,0.00001260625,0.00009260477,0.9985693,0.0001093531,0.00002368769,0.001054792],"study_design_scores_gemma":[0.000005754511,0.00006054813,0.0001915172,0.000002731778,0.000003532976,0.0001401517,0.000007945626,0.0004928169,0.9969952,0.00004688825,0.002048319,0.000004500922],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9396234,0.00109184,0.05474654,0.0001874372,0.00007944283,0.0001983482,0.0003900946,0.0003464983,0.003336485],"genre_scores_gemma":[0.9542438,0.0009461339,0.03806266,0.000136207,0.00002341152,0.0002036059,0.0005436211,0.0001271832,0.005713182],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000885049,"threshold_uncertainty_score":0.002960742,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04367484739463987,"score_gpt":0.2367730907285083,"score_spread":0.1930982433338684,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}