{"id":"W2796100013","doi":"10.1111/pbi.12928","title":"Recombinant protein susceptibility to proteolysis in the plant cell secretory pathway is <scp>pH</scp>‐dependent","year":2018,"lang":"en","type":"article","venue":"Plant Biotechnology Journal","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"Medicago (Canada); Université Laval","funders":"Fonds de recherche du Québec – Nature et technologies; Natural Sciences and Engineering Research Council of Canada","keywords":"Protease; Biology; Cystatin; Proteolysis; Secretory pathway; Biochemistry; Fusion protein; Nicotiana benthamiana; Cell biology; Proteases; Cysteine protease; Secretory protein; Recombinant DNA; Secretion; Golgi apparatus; Enzyme; Cell; Gene; Cystatin C","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003223541,0.0003314416,0.0002200755,0.0001170483,0.0001108206,0.0004628867,0.0001507917,0.0002673508,0.0005781366],"category_scores_gemma":[0.0002853732,0.0001417198,0.0002342559,0.0001338351,0.0002579417,0.0002791343,0.0002265186,0.0004213025,0.0002665175],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002845345,"about_ca_system_score_gemma":0.0001267318,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002642784,"about_ca_topic_score_gemma":0.000357212,"domain_scores_codex":[0.9997297,0.00005884509,0.00003061158,0.00006585312,0.00008143322,0.0000335495],"domain_scores_gemma":[0.9996821,0.00008844198,0.0001147048,0.00004708182,0.00003899823,0.00002866596],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001378916,0.000003270663,0.0001106245,0.000009820174,0.000002497623,0.000009974401,0.000004287981,0.00001427142,0.9995834,0.00001065531,0.000003521638,0.000233781],"study_design_scores_gemma":[9.800394e-7,0.00005330003,0.002062653,0.000001200021,0.000004272467,0.000131114,0.000006815771,0.0002522122,0.997173,0.00001037916,0.0003022116,0.000001840691],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9912646,0.0007621511,0.007123046,0.00005777799,0.000008342313,0.00002250278,0.0001983613,0.00006061079,0.0005025025],"genre_scores_gemma":[0.99611,0.0003634432,0.002487309,0.00003550313,0.000003862602,0.00001798702,0.0003658898,0.00003136522,0.0005846394],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005781366,"threshold_uncertainty_score":0.002064466,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01275069172145784,"score_gpt":0.2193540726943157,"score_spread":0.2066033809728579,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}