{"id":"W279622193","doi":"10.1007/s12010-015-1662-7","title":"A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass","year":2015,"lang":"en","type":"article","venue":"Applied Biochemistry and Biotechnology","topic":"Biofuel production and bioconversion","field":"Engineering","cited_by":30,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"National Renewable Energy Laboratory; Natural Sciences and Engineering Research Council of Canada","keywords":"Ninhydrin; Chemistry; Lignocellulosic biomass; Hydrolysis; Chromatography; Enzymatic hydrolysis; Biomass (ecology); Cellulase; Enzyme; Biochemistry; Monosaccharide; Amino acid; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008315302,0.001915229,0.0005175267,0.0009618376,0.0007771938,0.0003914019,0.001315387,0.0007455663,0.001183051],"category_scores_gemma":[0.0004722328,0.0007268275,0.0006193264,0.0006440464,0.0007494336,0.0005945236,0.0006052935,0.001483138,0.0008230421],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005953701,"about_ca_system_score_gemma":0.001066745,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002125471,"about_ca_topic_score_gemma":0.006895092,"domain_scores_codex":[0.9990225,0.000186496,0.0000971108,0.0001839433,0.0003913918,0.000118665],"domain_scores_gemma":[0.9995095,0.00009928591,0.00008513911,0.00008270986,0.0001466871,0.00007662378],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003810464,0.00002179177,0.00007415562,0.00004791519,0.000006962293,0.000015539,0.00001381515,0.00002346091,0.9988137,0.00004569009,0.00003040033,0.0008685447],"study_design_scores_gemma":[0.000004151259,0.00007632432,0.001103718,0.000006934464,0.00001523573,0.000096148,0.00001049626,0.0007252481,0.9971722,0.00002959981,0.0007537537,0.000006295551],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5651904,0.005906137,0.4136682,0.0005125543,0.0004320441,0.0006945521,0.001971186,0.00206734,0.00955766],"genre_scores_gemma":[0.5887991,0.004881676,0.3773462,0.0003611901,0.0001070511,0.0009553601,0.006653318,0.0002591187,0.02063702],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002125471,"threshold_uncertainty_score":0.004397571,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009870347105602126,"score_gpt":0.1935933540507136,"score_spread":0.1837230069451115,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}