{"id":"W2796337742","doi":"10.1186/s12915-018-0554-z","title":"Real-time tracking of complex ubiquitination cascades using a fluorescent confocal on-bead assay","year":2018,"lang":"en","type":"article","venue":"BMC Biology","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute for Research in Immunology and Cancer; Université de Montréal; Lunenfeld-Tanenbaum Research Institute; Mount Sinai Hospital","funders":"Medical Research Council; FP7 Research infrastructures; Wellcome Trust; Wellcome","keywords":"Ubiquitin; Biology; Proteasome; Cell biology; Enzyme; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007429414,0.0007801957,0.0005824366,0.0005966506,0.0004071896,0.0006731151,0.001129854,0.001064168,0.00188442],"category_scores_gemma":[0.0004925979,0.0003387753,0.0005824166,0.0004266642,0.0006204028,0.0004533499,0.0006747353,0.001060326,0.0008222954],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001447251,"about_ca_system_score_gemma":0.0007109898,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001564454,"about_ca_topic_score_gemma":0.002344648,"domain_scores_codex":[0.9988523,0.0001501521,0.00006722399,0.0004180774,0.0003531426,0.0001591052],"domain_scores_gemma":[0.9994121,0.0001729228,0.0001125608,0.00009919645,0.000129373,0.00007380215],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009331733,0.00005039578,0.0003290759,0.00005395717,0.00001277824,0.00003217987,0.00002038659,0.0004720396,0.9957444,0.0002714388,0.0001585236,0.002761373],"study_design_scores_gemma":[0.000008770458,0.00009192383,0.001096623,0.000004061222,0.00001388707,0.00008068464,0.00001101217,0.01232216,0.9851621,0.00006933602,0.001122134,0.00001731558],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5859769,0.001103445,0.4039839,0.000359934,0.00009409213,0.0004828669,0.001527381,0.001944208,0.00452729],"genre_scores_gemma":[0.5691603,0.00143137,0.421793,0.0002168541,0.00005225225,0.0009364087,0.001307059,0.0001253981,0.004977412],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00188442,"threshold_uncertainty_score":0.01050055,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06207238580197239,"score_gpt":0.327318495577302,"score_spread":0.2652461097753296,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}