{"id":"W2801216478","doi":"10.3791/57357","title":"Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Natural Science Foundation of China; ShanghaiTech University; U.S. Department of Veterans Affairs","keywords":"Phage display; Monoclonal antibody; Oligonucleotide; Immunogenicity; Electroporation; Hybridoma technology; Computational biology; Phagemid; Antibody; Peptide library; Molecular biology; Biology; DNA; Mutagenesis; Antigen; Chemistry; Genetics; Peptide sequence; Bacteriophage; Mutation; Escherichia coli; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003726567,0.0004666632,0.0005188885,0.0005783141,0.0002801146,0.0003731443,0.0004712883,0.0003744626,0.001467134],"category_scores_gemma":[0.0002629185,0.0002722548,0.0003860336,0.0007021052,0.0001603189,0.0002366332,0.0004226934,0.0005271517,0.0008153159],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002826446,"about_ca_system_score_gemma":0.0002132817,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001706935,"about_ca_topic_score_gemma":0.0002661057,"domain_scores_codex":[0.9997168,0.00005743567,0.00002414182,0.00005976507,0.0000763213,0.00006546355],"domain_scores_gemma":[0.9999173,0.00001330097,0.00001287726,0.00001192064,0.00002206954,0.00002255385],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007444251,0.00004671449,0.00009289487,0.00007229211,0.000009297964,0.0001012011,0.00003427755,0.0004336439,0.9942653,0.00026398,0.0001393515,0.004466719],"study_design_scores_gemma":[0.00002818986,0.000345328,0.0005264971,0.000008391299,0.00002899913,0.0002719841,0.00001891907,0.002317165,0.9895052,0.0001185324,0.006815329,0.00001539436],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8531412,0.00190045,0.1329305,0.0001914244,0.0001109132,0.0005911514,0.002054387,0.001003841,0.008075911],"genre_scores_gemma":[0.8805751,0.001703935,0.09899102,0.0002110513,0.00002853736,0.0005600112,0.00647545,0.0001920187,0.01126283],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001467134,"threshold_uncertainty_score":0.004908085,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03234267573541261,"score_gpt":0.4054443786477059,"score_spread":0.3731017029122933,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}