{"id":"W2802786597","doi":"10.3791/57431","title":"Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Zebrafish Biomedical Research Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"Cancer Research UK","keywords":"Microglia; Zebrafish; Biology; Cell biology; RNA; RNA extraction; Transcriptome; Cell culture; Homogenization (climate); Gene expression; Gene; Immunology; Inflammation; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001005909,0.00159916,0.001617856,0.003064469,0.002122293,0.0006565158,0.001195428,0.0009815403,0.005447991],"category_scores_gemma":[0.000889456,0.0009066319,0.001122911,0.001417699,0.001225362,0.000518135,0.001054287,0.002249996,0.004514616],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000602992,"about_ca_system_score_gemma":0.001869595,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002741265,"about_ca_topic_score_gemma":0.00769097,"domain_scores_codex":[0.9990024,0.00005462388,0.0001329933,0.0003029937,0.0003609718,0.0001460243],"domain_scores_gemma":[0.9994826,0.00007784386,0.00007118539,0.00009717091,0.0002047361,0.00006653063],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009442162,0.00003421097,0.0003599008,0.0004982869,0.00001621426,0.0004118837,0.0001622859,0.000166596,0.9916653,0.0004381798,0.0006675517,0.005485209],"study_design_scores_gemma":[0.0002150881,0.001332631,0.02852376,0.0006572318,0.0003337447,0.002088083,0.0003648806,0.002611502,0.826741,0.001896326,0.1350573,0.0001784507],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3601149,0.02121322,0.4389833,0.000929789,0.002000742,0.02599559,0.110239,0.00898358,0.03153973],"genre_scores_gemma":[0.2030171,0.0171479,0.6191984,0.001122423,0.000728295,0.03441508,0.09595381,0.002333813,0.02608304],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005447991,"threshold_uncertainty_score":0.01822531,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01880235002150542,"score_gpt":0.4121737228045467,"score_spread":0.3933713727830413,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}