{"id":"W2802786597","doi":"10.3791/57431","title":"Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Zebrafish Biomedical Research Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"Cancer Research UK","keywords":"Microglia; Zebrafish; Biology; Cell biology; RNA; RNA extraction; Transcriptome; Cell culture; Homogenization (climate); Gene expression; Gene; Immunology; Inflammation; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000191893,0.00008763096,0.0001477036,0.00006711937,0.00006043707,0.00002936907,0.0001033754,0.00009210823,0.00005133883],"category_scores_gemma":[0.0001862558,0.00007891661,0.00004256042,0.0000622841,0.000219418,0.00001706462,0.00008423383,0.0000842404,0.000001922904],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001100256,"about_ca_system_score_gemma":0.00004797447,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002636299,"about_ca_topic_score_gemma":0.000003516439,"domain_scores_codex":[0.9991149,0.00008251299,0.0003092379,0.0001546344,0.000213753,0.0001249913],"domain_scores_gemma":[0.9992816,0.00003138767,0.0002550095,0.0001212768,0.0001652373,0.0001454997],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002590929,0.0001119048,0.0008595512,0.000005217905,0.00006433619,0.00000223715,0.0001119174,3.854929e-8,0.9945328,0.00001227557,0.001626998,0.002413641],"study_design_scores_gemma":[0.001316652,0.0006763966,0.01372198,0.00002133391,0.00001699075,0.00002575527,0.0001212029,0.00004926999,0.9774568,0.0001131236,0.006412835,0.00006767148],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.994731,0.001426506,0.003367241,0.0001853241,0.00008982334,0.00009558263,0.00002145437,0.000002559598,0.00008047355],"genre_scores_gemma":[0.9941906,0.0005450247,0.004717591,0.00015457,0.0003005662,0.000003368475,0.000016769,0.00001270269,0.0000587516],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.017076,"threshold_uncertainty_score":0.3218126,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01880235002150542,"score_gpt":0.4121737228045467,"score_spread":0.3933713727830413,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}