{"id":"W2802861364","doi":"10.1039/c8md00090e","title":"Isolation, expression and biochemical characterization of recombinant hyoscyamine-6β-hydroxylase from <i>Brugmansia sanguinea</i> – tuning the scopolamine production","year":2018,"lang":"en","type":"article","venue":"MedChemComm","topic":"Plant tissue culture and regeneration","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Calgary Laboratory Services; University of Calgary; Alberta Hospital Edmonton; University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; National Research Foundation of Korea; Deutsche Forschungsgemeinschaft; National Research Foundation","keywords":"Hyoscyamine; Scopolamine; Isolation (microbiology); Recombinant DNA; Biology; Traditional medicine; Botany; Pharmacology; Biochemistry; Microbiology; Medicine; Solanaceae","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001242132,0.0001187581,0.0001131533,0.00002179101,0.0001353323,0.00001561529,0.00009720273,0.0001197133,0.00001209421],"category_scores_gemma":[0.00009558156,0.00008116422,0.00002591951,0.00007770487,0.0001425187,0.00001110384,0.0000657115,0.00006048248,0.000002909996],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006885891,"about_ca_system_score_gemma":0.00002303839,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006207141,"about_ca_topic_score_gemma":0.00006629011,"domain_scores_codex":[0.9992896,0.00005565169,0.0001972445,0.0002559331,0.00009522334,0.0001063914],"domain_scores_gemma":[0.999449,0.000008563231,0.0001301692,0.0002617263,0.0001138794,0.00003671803],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005177824,0.00003570638,0.0007281217,0.000009873689,0.00001422152,2.783266e-7,0.0002895204,4.624123e-7,0.9935513,0.000006236163,0.004557865,0.0007546577],"study_design_scores_gemma":[0.0002287849,0.00009619916,0.002049781,0.00005279288,0.00002145839,0.00001240267,0.0000453409,0.0002270609,0.9812138,0.00001850201,0.01592971,0.0001041781],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9957516,0.0007020186,0.002150302,0.0007356154,0.0004032867,0.0001589347,0.00001773824,0.00001192786,0.00006858802],"genre_scores_gemma":[0.9959151,0.0001626592,0.0006415731,0.00009471924,0.0009502189,0.00001160705,0.001541464,0.00001315152,0.0006695205],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01233749,"threshold_uncertainty_score":0.3309781,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0077267938162808,"score_gpt":0.2188665497346788,"score_spread":0.211139755918398,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}