{"id":"W2803265609","doi":"10.1021/acssynbio.8b00100","title":"Genetic Code Expansion in <i>Rhodobacter sphaeroides</i> to Incorporate Noncanonical Amino Acids into Photosynthetic Reaction Centers","year":2018,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Photosynthetic Processes and Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Division of Molecular and Cellular Biosciences; University of British Columbia; Oregon State University","keywords":"Rhodobacter sphaeroides; Amino acid; Genetic code; Photosynthesis; Photosynthetic reaction centre; Plasmid; Rhodobacter; Biology; Chemistry; Biochemistry; Gene; Mutant","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004562009,0.0004251259,0.0004399762,0.0001954502,0.0001391904,0.00002971662,0.0005855978,0.0005250879,0.00006602516],"category_scores_gemma":[0.0002776134,0.0003937047,0.0001161129,0.0003023098,0.0003895189,0.00001125842,0.0004004836,0.0002229005,0.0002213392],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008773506,"about_ca_system_score_gemma":0.000197556,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002953025,"about_ca_topic_score_gemma":0.001032418,"domain_scores_codex":[0.9971105,0.0002390461,0.0006002986,0.001108555,0.0001618905,0.000779703],"domain_scores_gemma":[0.9984365,0.0000496243,0.0001898966,0.0008726391,0.0001633118,0.0002880481],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003971562,0.0001517805,0.001041777,0.00001907426,0.00002589117,0.00001026598,0.0001477426,0.000004078448,0.9879526,0.0001032846,0.00008641406,0.01005995],"study_design_scores_gemma":[0.0006906479,0.001406938,0.001332954,0.00008345168,0.00003053179,0.0001310849,0.00008520054,0.00005918569,0.9600239,0.001409383,0.03427155,0.0004751353],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9892972,0.0001130545,0.007885426,0.0005148752,0.0007901725,0.000530479,0.00003707721,0.00004461012,0.0007871102],"genre_scores_gemma":[0.9947457,0.0002352147,0.002465724,0.001778961,0.0003312012,0.0001516627,0.00006112842,0.00007011736,0.000160315],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03418514,"threshold_uncertainty_score":0.9998515,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01090728262614927,"score_gpt":0.2609377223987567,"score_spread":0.2500304397726074,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}