{"id":"W2805214660","doi":"10.1093/glycob/cwy051","title":"Conserved residues Arg188 and Asp302 are critical for active site organization and catalysis in human ABO(H) blood group A and B glycosyltransferases†","year":2018,"lang":"en","type":"article","venue":"Glycobiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta; University of Victoria","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Alberta Glycomics Centre","keywords":"Glycosyltransferase; Chemistry; Salt bridge; Active site; Stereochemistry; Enzyme; Mutant; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001974496,0.0001661975,0.000235275,0.0001188294,0.000209613,0.00004011811,0.00007462817,0.0002747003,0.00003445821],"category_scores_gemma":[0.0006003154,0.0001607979,0.00002390355,0.0001461403,0.0005800538,0.00001159158,0.000104151,0.0001012746,0.000001965954],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008267432,"about_ca_system_score_gemma":0.00003141663,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001520124,"about_ca_topic_score_gemma":0.002698754,"domain_scores_codex":[0.9987613,0.0001327469,0.0002122505,0.0005353674,0.00005768575,0.0003006361],"domain_scores_gemma":[0.9993576,0.00009792679,0.00004215323,0.0001841564,0.0002073551,0.0001108181],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004465767,0.0000962148,0.06403127,0.00007022636,0.00005967533,0.000008856008,0.0001871995,1.735345e-7,0.9330948,0.001096674,0.0001244475,0.0007838634],"study_design_scores_gemma":[0.005656967,0.00342949,0.1932782,0.00005411001,0.0001192684,0.0002327175,0.0003947724,0.00007960793,0.7893218,0.001261291,0.005592771,0.0005789184],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9977633,0.0005799728,0.000587387,0.0004559993,0.00003521406,0.0003812487,0.0001053443,0.00001353114,0.00007799749],"genre_scores_gemma":[0.9983448,0.0002868892,0.0003710101,0.0002191528,0.0001487727,0.00004910141,0.0004903378,0.00002115453,0.0000687908],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.143773,"threshold_uncertainty_score":0.6557147,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01102904613393961,"score_gpt":0.2866169469959536,"score_spread":0.275587900862014,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}