{"id":"W2805214660","doi":"10.1093/glycob/cwy051","title":"Conserved residues Arg188 and Asp302 are critical for active site organization and catalysis in human ABO(H) blood group A and B glycosyltransferases†","year":2018,"lang":"en","type":"article","venue":"Glycobiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta; University of Victoria","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Alberta Glycomics Centre","keywords":"Glycosyltransferase; Chemistry; Salt bridge; Active site; Stereochemistry; Enzyme; Mutant; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001075386,0.0004342803,0.0003654538,0.00007686735,0.0001983621,0.0003370834,0.0002039383,0.0003401356,0.001563215],"category_scores_gemma":[0.0001846391,0.0001641362,0.0002438907,0.000110576,0.000183924,0.0002423155,0.0001284689,0.0003758268,0.0007674294],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003305694,"about_ca_system_score_gemma":0.0002491703,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00113894,"about_ca_topic_score_gemma":0.0009012385,"domain_scores_codex":[0.9999042,0.0000141044,0.000006612867,0.0000231372,0.00003355423,0.00001842974],"domain_scores_gemma":[0.9998599,0.00001602474,0.00005335169,0.000007084332,0.00001050192,0.00005299975],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007752377,0.0001010328,0.003184045,0.00006578852,0.00001580927,0.0008609502,0.00006845721,0.001224289,0.9901218,0.0003439729,0.0002342861,0.003004347],"study_design_scores_gemma":[0.0002092574,0.0008214624,0.04638025,0.00002652998,0.00004413349,0.004700572,0.0001822759,0.01288048,0.9266058,0.0004459485,0.007646131,0.00005708488],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9968232,0.0004086799,0.001924055,0.00006242321,0.00001782628,0.00001319649,0.0001923399,0.00005008284,0.0005082195],"genre_scores_gemma":[0.9964576,0.0001671246,0.00201794,0.00002745916,0.000005294844,0.000009668387,0.0005131213,0.00002039051,0.000781273],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001563215,"threshold_uncertainty_score":0.005229473,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01102904613393961,"score_gpt":0.2866169469959536,"score_spread":0.275587900862014,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}