{"id":"W2806213267","doi":"10.1007/978-1-4939-7871-7_6","title":"A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning","year":2018,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":21,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Centre National de la Recherche Scientifique; Institut national de la recherche scientifique; National Institute of General Medical Sciences; University of South Dakota; University of Nebraska Medical Center","keywords":"Adenylate kinase; Cyclase; Operon; Adenylate Cyclase Toxin; lac operon; Gene; Bordetella pertussis; Cloning (programming); Biology; Biochemistry; Cell biology; Gateway (web page); Chemistry; G protein; Escherichia coli; Genetics; Pertussis toxin; Bacteria; Enzyme; Signal transduction; Computer science","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001985693,0.003182612,0.004018085,0.002688874,0.001271503,0.002319043,0.003951941,0.002338717,0.01035092],"category_scores_gemma":[0.0009488608,0.002314927,0.002387835,0.002292267,0.001541334,0.001255816,0.002282065,0.005705623,0.01590083],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00130807,"about_ca_system_score_gemma":0.001211041,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001198821,"about_ca_topic_score_gemma":0.00185748,"domain_scores_codex":[0.9964037,0.0006077454,0.0003665058,0.001109976,0.001066647,0.0004453698],"domain_scores_gemma":[0.9993294,0.0002138157,0.00008917553,0.0001516031,0.00005635951,0.0001595957],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002813089,0.0001112863,0.00005840376,0.000455569,0.0000371409,0.00008726719,0.00008419001,0.0001276033,0.9907076,0.001157407,0.00130314,0.005589086],"study_design_scores_gemma":[0.000274538,0.0004522328,0.0009461725,0.00009271815,0.000184939,0.0006267058,0.00003549699,0.001877885,0.9157384,0.001097333,0.07851048,0.0001630516],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1706183,0.007386305,0.7126578,0.001613904,0.003034438,0.008461325,0.03600383,0.03606205,0.02416207],"genre_scores_gemma":[0.2421299,0.007212786,0.561458,0.001100843,0.000413521,0.006471138,0.09445488,0.005538287,0.08122066],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.01035092,"threshold_uncertainty_score":0.03462726,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01256649121183166,"score_gpt":0.3247195395860345,"score_spread":0.3121530483742028,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}