{"id":"W2806748964","doi":"10.1371/journal.pone.0197956","title":"Design of a glutamine substrate tag enabling protein labelling mediated by Bacillus subtilis transglutaminase","year":2018,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Bacillus subtilis; Tissue transglutaminase; Glutamine; Labelling; Biochemistry; Substrate (aquarium); Chemistry; Substrate specificity; Enzyme; Biology; Amino acid; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004746689,0.0001596361,0.0002176661,0.00003853043,0.00007386656,0.00001397103,0.000206894,0.0001681088,0.0001018575],"category_scores_gemma":[0.0002812082,0.0001587602,0.00004408999,0.0001755479,0.0002146044,0.000006423934,0.00002454564,0.00009273748,0.0000178847],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001379988,"about_ca_system_score_gemma":0.00008123416,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002154605,"about_ca_topic_score_gemma":0.0000139227,"domain_scores_codex":[0.9986481,0.0001422059,0.0003440471,0.0003790125,0.0002286845,0.0002579606],"domain_scores_gemma":[0.9990286,0.00002246789,0.0001290468,0.0004187838,0.0002971988,0.0001038651],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002379546,0.000742586,0.0001470106,0.00009160102,0.0001027301,8.639568e-7,0.0001022491,0.000002803261,0.9980031,0.000009619926,0.00001630981,0.0005432308],"study_design_scores_gemma":[0.0006273356,0.0005295692,0.00009255969,0.0000473231,0.00003928657,5.811214e-7,0.00002851999,0.0008448734,0.9971184,0.00003858721,0.000459343,0.0001736514],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9781749,0.001614586,0.01902883,0.0001390204,0.00001202401,0.00076864,0.0001054129,0.00002846818,0.0001280894],"genre_scores_gemma":[0.9940885,0.0001549212,0.004962597,0.0000464495,0.00007061541,0.00009423123,0.0001751034,0.00002306836,0.0003845422],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01591354,"threshold_uncertainty_score":0.6474053,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03997340099806552,"score_gpt":0.2278479275170536,"score_spread":0.1878745265189881,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}