{"id":"W2806774268","doi":"10.1016/j.jbiotec.2018.06.307","title":"Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells","year":2018,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":53,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Research Council Canada","funders":"U.S. Department of Energy","keywords":"Chinese hamster ovary cell; Transfection; Polyethylenimine; Recombinant DNA; Cell culture; Molecular biology; clone (Java method); Biology; Chemistry; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001444447,0.001056037,0.001223378,0.0006473443,0.0006114259,0.0008426411,0.0008923142,0.0007643452,0.001020045],"category_scores_gemma":[0.0006987196,0.0005127643,0.0009721641,0.0007769787,0.0003736463,0.0004823647,0.0004335375,0.001164791,0.001236461],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007334023,"about_ca_system_score_gemma":0.0006062141,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001577555,"about_ca_topic_score_gemma":0.002850018,"domain_scores_codex":[0.9988678,0.0002183072,0.0002061847,0.0002160552,0.0003705747,0.0001211769],"domain_scores_gemma":[0.9996316,0.0001147994,0.00006177515,0.00005840537,0.00009404327,0.00003942375],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002946941,0.00002859645,0.00005457013,0.00004674776,0.000006546989,0.0000212514,0.00001768658,0.00009995707,0.9984019,0.00006002692,0.00004068983,0.001192469],"study_design_scores_gemma":[0.00001379859,0.00007402011,0.0006652449,0.000004648795,0.00002625216,0.00007292488,0.00001047411,0.00101702,0.9955663,0.00002837669,0.002507465,0.00001357262],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6472105,0.002276957,0.3410262,0.0004583849,0.0003316023,0.001879288,0.002125803,0.001682386,0.003008831],"genre_scores_gemma":[0.4394624,0.002416595,0.5401256,0.0002561891,0.00007808472,0.002498306,0.006920516,0.0008427598,0.007399515],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001577555,"threshold_uncertainty_score":0.00763905,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006233221398554097,"score_gpt":0.2532897441108898,"score_spread":0.2470565227123357,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}