{"id":"W2806774268","doi":"10.1016/j.jbiotec.2018.06.307","title":"Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells","year":2018,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":53,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Research Council Canada","funders":"U.S. Department of Energy","keywords":"Chinese hamster ovary cell; Transfection; Polyethylenimine; Recombinant DNA; Cell culture; Molecular biology; clone (Java method); Biology; Chemistry; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003800025,0.000114709,0.000228191,0.00024435,0.00005236373,0.000005693521,0.000121176,0.0003896626,0.00001534923],"category_scores_gemma":[0.0001359601,0.0001018544,0.0001233798,0.0002040014,0.0001058403,0.000009821979,0.00002749309,0.0001388147,4.874844e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002424048,"about_ca_system_score_gemma":0.00006755579,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003727822,"about_ca_topic_score_gemma":0.00003178603,"domain_scores_codex":[0.999037,0.00008851392,0.0004160958,0.0002149362,0.00008806505,0.0001553563],"domain_scores_gemma":[0.9991381,0.000007402362,0.0003745958,0.0001920777,0.0002510923,0.00003678662],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007949672,0.0002383299,0.00002717186,0.00004828085,0.0000294364,9.139553e-7,0.00002634614,0.003008618,0.9869599,0.00001892096,0.0006765874,0.008170563],"study_design_scores_gemma":[0.00115281,0.003101087,0.00008183967,0.00004064872,0.0000287622,0.00004161747,0.00004050715,0.0003872633,0.9936895,0.000228555,0.001108278,0.00009915637],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7221705,0.0001995093,0.2762579,0.0005017217,0.0004230859,0.0004220403,0.000006772836,0.000007735337,0.00001075959],"genre_scores_gemma":[0.9652365,0.0002503459,0.03398044,0.0000363038,0.0003826951,0.00002473057,0.000007433811,0.00001516705,0.00006636585],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2430661,"threshold_uncertainty_score":0.4153503,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006233221398554097,"score_gpt":0.2532897441108898,"score_spread":0.2470565227123357,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}