{"id":"W2807389226","doi":"10.3390/mps1020017","title":"A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies","year":2018,"lang":"en","type":"article","venue":"Methods and Protocols","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"Saskatchewan Research Council (Canada)","funders":"","keywords":"Cas9; Genome editing; Ribonucleoprotein; Computational biology; CRISPR; Nuclease; RNA; DNA; Genome; Biology; Gene; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006910033,0.001239083,0.00101273,0.001160478,0.001109084,0.0005782229,0.001457927,0.001010529,0.003316777],"category_scores_gemma":[0.0006076787,0.0007920741,0.0007783326,0.0008178615,0.000457044,0.0007065632,0.001050341,0.002103723,0.004828569],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005081064,"about_ca_system_score_gemma":0.001073227,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009049666,"about_ca_topic_score_gemma":0.001795088,"domain_scores_codex":[0.9985055,0.0001188526,0.0001693349,0.0003324747,0.0007604682,0.0001133529],"domain_scores_gemma":[0.9996544,0.00005925817,0.00004851997,0.00009687752,0.00009449357,0.000046402],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005454686,0.00009919234,0.0001434098,0.000386023,0.00002182502,0.0001734379,0.00006596898,0.0001428257,0.9779767,0.0008964811,0.002206421,0.0178331],"study_design_scores_gemma":[0.0000246068,0.0001345699,0.0009652379,0.00002864835,0.00003845528,0.001074473,0.00002236493,0.001460688,0.92349,0.0003350816,0.07234482,0.00008109406],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05864154,0.005357602,0.9133797,0.0006609676,0.0007420991,0.003819693,0.002962372,0.004778584,0.009657479],"genre_scores_gemma":[0.1090526,0.005956329,0.834542,0.0005772976,0.0001177025,0.005965733,0.009943072,0.0007705156,0.03307479],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003316777,"threshold_uncertainty_score":0.01109576,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03954153246290205,"score_gpt":0.4908049222223368,"score_spread":0.4512633897594348,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}