{"id":"W2807624799","doi":"10.1038/nbt.4166","title":"Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos","year":2018,"lang":"en","type":"article","venue":"Nature Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":347,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; Hospital for Sick Children","funders":"","keywords":"CRISPR; Biology; Homologous recombination; Genome editing; Gene targeting; Transgenesis; Gene knockin; Genetics; Chromatin; Cas9; Embryo; Computational biology; Gene; Cell biology; Embryogenesis; Reproductive technology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009565197,0.0001390557,0.0001189615,0.0001231939,0.00009174777,0.000006304308,0.000157221,0.0008869574,0.00001947525],"category_scores_gemma":[0.00006171639,0.0001410755,0.00005588866,0.00020562,0.0001017773,0.000001279125,0.0000948732,0.0002703292,0.000008402749],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001997234,"about_ca_system_score_gemma":0.00002838886,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002407499,"about_ca_topic_score_gemma":0.0001202876,"domain_scores_codex":[0.9991708,0.00002103168,0.000184563,0.0003304257,0.00007820364,0.000215],"domain_scores_gemma":[0.9994131,0.000003060275,0.00007148677,0.0003717958,0.0001063517,0.00003417144],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002051873,0.00009264134,0.00009099358,0.0000112334,0.0000186017,3.479027e-7,0.00002708852,0.0002610383,0.9946691,0.0001747922,0.004313237,0.0003204093],"study_design_scores_gemma":[0.0004936097,0.0002959017,0.00005335365,0.000002817863,0.00001043351,0.000004079443,0.00003692431,0.003373171,0.9665615,0.000004442785,0.02902548,0.0001382685],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9416199,0.002437076,0.05504516,0.0002086041,0.0003342722,0.000155685,0.00005758763,0.0000548102,0.00008685188],"genre_scores_gemma":[0.9957168,0.00007858736,0.002906515,0.0001250578,0.0001845768,0.00001027191,0.0002334299,0.00002142575,0.0007233228],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05409686,"threshold_uncertainty_score":0.6841028,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00329830535719781,"score_gpt":0.2674415670147394,"score_spread":0.2641432616575416,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}