{"id":"W2808880569","doi":"10.1534/g3.118.200539","title":"A <i>Drosophila</i> CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues","year":2018,"lang":"en","type":"article","venue":"G3 Genes Genomes Genetics","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"CRISPR; Cas9; Biology; Polytene chromosome; Prothoracic gland; RNA interference; Gene; Computational biology; Gene expression; Regulation of gene expression; Phenotype; Genome editing; Cell biology; Genetics; Drosophila melanogaster; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003758799,0.0003392597,0.0002497538,0.00008623979,0.0003151844,0.00009641839,0.0003667142,0.0002134628,0.00001546907],"category_scores_gemma":[0.00005680016,0.0002932907,0.0001491655,0.000143506,0.0001074653,0.000006754222,0.0001328693,0.00007981361,0.000007851324],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002106044,"about_ca_system_score_gemma":0.00009512236,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001088095,"about_ca_topic_score_gemma":0.0001203197,"domain_scores_codex":[0.998151,0.00004802771,0.0004510972,0.000610586,0.0001917136,0.0005475893],"domain_scores_gemma":[0.9988331,0.0001170438,0.0001305132,0.0005836864,0.0002311663,0.0001044789],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001361581,0.0001047256,0.00272426,0.0001349673,0.00004115658,0.00004416757,0.000447484,0.00143069,0.9421175,0.00002682405,0.0006649758,0.0521271],"study_design_scores_gemma":[0.001334683,0.001473447,0.0005694788,0.00003327768,0.00007363603,0.001136478,0.0004247441,0.002484913,0.8488398,0.0004494845,0.1426406,0.0005394492],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.754523,0.164381,0.07580749,0.0004263902,0.0004223394,0.00366056,0.0004880237,0.00004273532,0.0002484604],"genre_scores_gemma":[0.9426986,0.003927725,0.04889791,0.0005238733,0.002123528,0.0009484133,0.0004665162,0.00009376621,0.0003196887],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1881756,"threshold_uncertainty_score":0.9999519,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02069106736975718,"score_gpt":0.3382022332804061,"score_spread":0.3175111659106489,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}