{"id":"W2808880569","doi":"10.1534/g3.118.200539","title":"A <i>Drosophila</i> CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues","year":2018,"lang":"en","type":"article","venue":"G3 Genes Genomes Genetics","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"CRISPR; Cas9; Biology; Polytene chromosome; Prothoracic gland; RNA interference; Gene; Computational biology; Gene expression; Regulation of gene expression; Phenotype; Genome editing; Cell biology; Genetics; Drosophila melanogaster; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003733362,0.0009211782,0.0004993113,0.0008077214,0.0004939382,0.0005825532,0.001273915,0.0007096914,0.01241633],"category_scores_gemma":[0.0002754172,0.0005754999,0.000584828,0.0004624299,0.0004273322,0.0004295165,0.000774479,0.001470606,0.01249807],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005048444,"about_ca_system_score_gemma":0.000458856,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001054274,"about_ca_topic_score_gemma":0.002475307,"domain_scores_codex":[0.9996588,0.00004044625,0.00005514808,0.00008162462,0.000123376,0.0000406183],"domain_scores_gemma":[0.9997296,0.00003791065,0.00006681198,0.00008385648,0.00003116727,0.00005058156],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009463962,0.00003093712,0.0001751059,0.0003027599,0.00002472045,0.0002231409,0.00004574828,0.0002096313,0.9665205,0.002414564,0.01224675,0.01771147],"study_design_scores_gemma":[0.00005506959,0.0001572321,0.002031303,0.00005582745,0.00005276199,0.001816578,0.00002338755,0.002600564,0.7738054,0.0005300534,0.2188144,0.00005735141],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1234602,0.004643122,0.647245,0.002073523,0.00150256,0.002396472,0.06073159,0.1087334,0.04921402],"genre_scores_gemma":[0.265169,0.004657731,0.5768687,0.001310294,0.0001745301,0.002593956,0.06540578,0.01321932,0.07060086],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01241633,"threshold_uncertainty_score":0.04153675,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02069106736975718,"score_gpt":0.3382022332804061,"score_spread":0.3175111659106489,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}