{"id":"W2809933263","doi":"10.1016/j.jbiotec.2018.07.006","title":"Enhanced overexpression, purification of a channelrhodopsin and a fluorescent flux assay for its functional characterization","year":2018,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Photoreceptor and optogenetics research","field":"Neuroscience","cited_by":3,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Institute for Nanotechnology; Alberta Glycomics Centre; University of Alberta","funders":"University of Alberta; Alberta Innovates; Alberta Innovates - Technology Futures","keywords":"Chlamydomonas reinhardtii; Channelrhodopsin; Optogenetics; Vesicle; Biophysics; Pichia pastoris; Chemistry; Recombinant DNA; Cell biology; Membrane; Fluorescence; Chlamydomonas; Flux (metallurgy); Green fluorescent protein; Biochemistry; Biology; Gene; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005329342,0.0004307018,0.0003788159,0.0003527104,0.0003139883,0.0005194804,0.0003423039,0.0004113198,0.0005907839],"category_scores_gemma":[0.0004248621,0.0002165876,0.0005452301,0.0004897903,0.0002800139,0.0004436374,0.0003621912,0.001023751,0.0005357956],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006253005,"about_ca_system_score_gemma":0.0004814028,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001898554,"about_ca_topic_score_gemma":0.001838741,"domain_scores_codex":[0.999694,0.00003687755,0.00004708741,0.00005296609,0.000104852,0.00006426077],"domain_scores_gemma":[0.9997306,0.00005986995,0.00004816024,0.00005575482,0.00006787714,0.00003762883],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001581999,0.00001771289,0.00005146093,0.00001738497,0.000003075448,0.00001761411,0.00001052795,0.0000362605,0.9992217,0.0001128367,0.00002573872,0.0004699114],"study_design_scores_gemma":[0.000004919981,0.00002972195,0.001231871,0.000003395358,0.00001253733,0.0001030372,0.00001314164,0.0008571607,0.9954597,0.00005832259,0.002221929,0.000004271709],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9067475,0.001762902,0.08421624,0.0007312758,0.0001660216,0.0002748529,0.003123973,0.0004243252,0.002552962],"genre_scores_gemma":[0.9169571,0.001877818,0.06327833,0.0001994135,0.0000429375,0.0002375971,0.007992161,0.0002298915,0.00918468],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001898554,"threshold_uncertainty_score":0.004536867,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04515585891095723,"score_gpt":0.3055630980903536,"score_spread":0.2604072391793964,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}