{"id":"W2810719551","doi":"10.3791/58024","title":"High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Zebrafish Biomedical Research Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"Children's Hospital of Eastern Ontario; University of Ottawa","funders":"Genetics Society of America; Genome Canada; Canadian Institutes of Health Research; University of Ottawa","keywords":"Zebrafish; Genotyping; Biology; Genotype; Genetics; Danio; DNA sequencing; Computational biology; genomic DNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004226548,0.0001160831,0.0002198017,0.0000803316,0.00008926987,0.0000285205,0.0001962781,0.0001353624,0.00009256077],"category_scores_gemma":[0.0002734377,0.000106042,0.00007430705,0.0001176325,0.0002343333,0.00002008064,0.0001130157,0.0001171748,0.000005319101],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003160238,"about_ca_system_score_gemma":0.00009264498,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002704265,"about_ca_topic_score_gemma":0.000002379028,"domain_scores_codex":[0.9986599,0.00009137153,0.0004879273,0.0001925369,0.0003481558,0.0002201185],"domain_scores_gemma":[0.9989551,0.00002975941,0.0003929328,0.0001734305,0.0002689666,0.0001798663],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002060317,0.0001786467,0.0001067597,0.00001212508,0.0001021405,0.000001492322,0.0001028051,2.141169e-7,0.9872097,0.00004797676,0.009972517,0.002059532],"study_design_scores_gemma":[0.00133097,0.0006731641,0.0005715291,0.00004560691,0.00001483978,0.00002458059,0.0001278033,0.00002496676,0.9582751,0.0001062326,0.03870955,0.0000956179],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9849828,0.00129957,0.0130798,0.0002246721,0.0001509001,0.0001293365,0.00001321509,0.000004327586,0.0001154036],"genre_scores_gemma":[0.9929576,0.0004811701,0.005774152,0.0002199882,0.0004141164,0.000008306212,0.00002469982,0.00001955685,0.0001004484],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02893462,"threshold_uncertainty_score":0.4324269,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02223824830390886,"score_gpt":0.4227176814301045,"score_spread":0.4004794331261956,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}