{"id":"W2810719551","doi":"10.3791/58024","title":"High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Zebrafish Biomedical Research Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"Children's Hospital of Eastern Ontario; University of Ottawa","funders":"Genetics Society of America; Genome Canada; Canadian Institutes of Health Research; University of Ottawa","keywords":"Zebrafish; Genotyping; Biology; Genotype; Genetics; Danio; DNA sequencing; Computational biology; genomic DNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00140843,0.001248007,0.001089326,0.002430722,0.001317652,0.0006152018,0.001371649,0.001322094,0.00380664],"category_scores_gemma":[0.001454823,0.001026888,0.001557507,0.0009752635,0.0007880605,0.0003257795,0.0007097026,0.001548681,0.004986609],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005437964,"about_ca_system_score_gemma":0.001332023,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.006203359,"about_ca_topic_score_gemma":0.02464989,"domain_scores_codex":[0.9979615,0.0001801046,0.0002141522,0.0006717555,0.0007963915,0.0001761769],"domain_scores_gemma":[0.9990547,0.0002071956,0.0001663105,0.0002167315,0.0002899253,0.00006517559],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003965482,0.0000263266,0.0003517019,0.00008935636,0.00001553575,0.0001188273,0.00005649336,0.0001425078,0.9922425,0.0001278447,0.0005671801,0.006222205],"study_design_scores_gemma":[0.00006870939,0.0004184291,0.0225222,0.0001117759,0.000180279,0.0007558182,0.0000617912,0.003892136,0.9382707,0.0004387078,0.03318167,0.00009773699],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1038656,0.0013904,0.8530517,0.0002998286,0.0003085018,0.006328742,0.02233482,0.006247782,0.006172503],"genre_scores_gemma":[0.09378167,0.002216418,0.8494018,0.0007014993,0.00009112532,0.008207026,0.03182618,0.001573785,0.01220061],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006203359,"threshold_uncertainty_score":0.01273453,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02223824830390886,"score_gpt":0.4227176814301045,"score_spread":0.4004794331261956,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}