{"id":"W2811107629","doi":"10.1002/cpns.49","title":"Assessing Individual Neuronal Activity Across the Intact Brain: Using Hybridization Chain Reaction (HCR) to Detect<i>Arc</i>mRNA Localized to the Nucleus in Volumes of Cleared Brain Tissue","year":2018,"lang":"en","type":"article","venue":"Current Protocols in Neuroscience","topic":"Neuroscience and Neuropharmacology Research","field":"Neuroscience","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; Canadian Institute for Advanced Research; Ontario Brain Institute; University of Toronto; SickKids Foundation; Hospital for Sick Children","funders":"National Institutes of Health; Canadian Institutes of Health Research; Hospital for Sick Children","keywords":"Clearance; Arc (geometry); Nucleus; Messenger RNA; In situ hybridization; Biology; Neuroscience; Pathology; Chemistry; Medicine; Gene; Biochemistry; Mathematics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaresearch","metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.003216734,0.0003715598,0.0003785812,0.0004943277,0.001094509,0.0007083587,0.002609932,0.00007835012,0.00001048592],"category_scores_gemma":[0.009520247,0.0002779497,0.00007490009,0.00578108,0.00159443,0.00138639,0.00140904,0.001101966,0.0000238826],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001648489,"about_ca_system_score_gemma":0.0004126296,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005588268,"about_ca_topic_score_gemma":0.0000923399,"domain_scores_codex":[0.9924588,0.002431888,0.0007085644,0.001579781,0.001544751,0.001276282],"domain_scores_gemma":[0.9972169,0.0012134,0.0003693035,0.0008164148,0.0001262275,0.0002577853],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002822111,0.0002384138,0.00134745,0.00003292552,3.198969e-7,0.00002693527,0.0008268792,0.0006362804,0.9514194,0.00002710332,0.0002092905,0.04495279],"study_design_scores_gemma":[0.0008205291,0.0009643221,0.05355881,0.0001647433,0.000002953693,0.00006598925,0.00005870813,0.0186877,0.9059085,0.00005297601,0.01942271,0.0002920882],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.964795,0.000002450559,0.001906606,0.003675159,0.001154479,0.02834154,0.00003925519,0.00006303001,0.00002248454],"genre_scores_gemma":[0.9883756,0.000005237615,0.00004679515,0.004994325,0.0001755176,0.006332665,2.876918e-7,0.00004270285,0.0000268678],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05221136,"threshold_uncertainty_score":0.9999673,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1865929602932421,"score_gpt":0.4940041160282296,"score_spread":0.3074111557349876,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}