{"id":"W2883800592","doi":"10.1016/j.omtm.2018.07.004","title":"Accurate Titration of Infectious AAV Particles Requires Measurement of Biologically Active Vector Genomes and Suitable Controls","year":2018,"lang":"en","type":"article","venue":"Molecular Therapy — Methods & Clinical Development","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":52,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Université de Nantes; Commissariat Général à l'Investissement; Centre hospitalier universitaire Sainte-Justine; Agence Nationale de la Recherche; Institut National de la Santé et de la Recherche Médicale; Association Française contre les Myopathies","keywords":"Genome; Biology; Infectivity; Vector (molecular biology); Virology; Viral vector; Intracellular; Computational biology; Transgene; Virus quantification; Virus; Recombinant DNA; Gene; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.01029287,0.002511219,0.001900145,0.001636236,0.0006629255,0.002185158,0.001174604,0.002100382,0.001543067],"category_scores_gemma":[0.01359353,0.001150069,0.0009686425,0.001135224,0.002163014,0.001477131,0.001200567,0.0028206,0.002312763],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001020215,"about_ca_system_score_gemma":0.001192155,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001085292,"about_ca_topic_score_gemma":0.001653422,"domain_scores_codex":[0.9839953,0.003845047,0.001665352,0.002664357,0.007204746,0.0006252032],"domain_scores_gemma":[0.990801,0.004745675,0.001145033,0.001044003,0.002016746,0.0002475395],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003627668,0.0001085032,0.0004649087,0.0002413663,0.00001035563,0.00002446043,0.0001076903,0.0002270517,0.9944844,0.000210613,0.00009391454,0.003990561],"study_design_scores_gemma":[0.00001159026,0.0002978259,0.002031138,0.00007218241,0.00003244747,0.0001151164,0.00005228779,0.001897416,0.9909658,0.0002700999,0.004211717,0.00004230754],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.220263,0.01016657,0.7560113,0.0005532306,0.0007923251,0.003216144,0.001308961,0.003018333,0.004670063],"genre_scores_gemma":[0.2938573,0.007139393,0.6787478,0.0008399639,0.0002526979,0.008093188,0.004947908,0.001036204,0.005085552],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01029287,"threshold_uncertainty_score":0.05443454,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1191263058718998,"score_gpt":0.4409644334031738,"score_spread":0.3218381275312741,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}