{"id":"W2883800592","doi":"10.1016/j.omtm.2018.07.004","title":"Accurate Titration of Infectious AAV Particles Requires Measurement of Biologically Active Vector Genomes and Suitable Controls","year":2018,"lang":"en","type":"article","venue":"Molecular Therapy — Methods & Clinical Development","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":52,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Université de Nantes; Commissariat Général à l'Investissement; Centre hospitalier universitaire Sainte-Justine; Agence Nationale de la Recherche; Institut National de la Santé et de la Recherche Médicale; Association Française contre les Myopathies","keywords":"Genome; Biology; Infectivity; Vector (molecular biology); Virology; Viral vector; Intracellular; Computational biology; Transgene; Virus quantification; Virus; Recombinant DNA; Gene; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004736475,0.000218805,0.0004649868,0.00006317189,0.00007901772,0.00001915557,0.0002345277,0.0002343928,0.00005270084],"category_scores_gemma":[0.0008807282,0.0001749092,0.0001400844,0.000156411,0.0004506207,0.000007896674,0.000123317,0.0001320277,0.000005080733],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003163404,"about_ca_system_score_gemma":0.0003694558,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000886241,"about_ca_topic_score_gemma":0.00004304526,"domain_scores_codex":[0.9963937,0.001609655,0.0008449405,0.000491266,0.0003340071,0.000326439],"domain_scores_gemma":[0.9983054,0.0001842901,0.0003355462,0.0003882198,0.000657928,0.0001286442],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002820555,0.000213098,0.001881858,0.00001118363,0.0004654446,0.000001082642,0.0001100282,0.000006829056,0.7989751,0.00004330916,0.00001381021,0.1954577],"study_design_scores_gemma":[0.001715792,0.005878685,0.01651707,0.00001908353,0.00001764705,0.000001533113,0.00002925872,0.0000321185,0.9701003,0.0004840704,0.00500884,0.0001955546],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9509133,0.003348666,0.04480805,0.0001145272,0.0001192672,0.0005423278,0.000006110075,0.00001279279,0.0001349639],"genre_scores_gemma":[0.9498175,0.0016825,0.0479945,0.0003049732,0.00007379884,0.00005906483,0.00001450076,0.00002482185,0.00002837326],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1952621,"threshold_uncertainty_score":0.713259,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1191263058718998,"score_gpt":0.4409644334031738,"score_spread":0.3218381275312741,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}