{"id":"W2885348010","doi":"10.1002/cbic.201800305","title":"Quick Click: The DNA‐Templated Ligation of 3′‐<i>O</i>‐Propargyl‐ and 5′‐Azide‐Modified Strands Is as Rapid as and More Selective than Ligase","year":2018,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Grand Challenges Canada; Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","keywords":"DNA ligase; Azide; Chemistry; Oligonucleotide; Click chemistry; Propargyl; Combinatorial chemistry; Cycloaddition; DNA; Ligation; Chemical ligation; Bioproduction; Stereochemistry; Biochemistry; Organic chemistry; Biology; Peptide; Molecular biology; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001491976,0.0002693631,0.0002741393,0.00002382106,0.0002862096,0.00005449497,0.0002330146,0.0002601082,0.0002527488],"category_scores_gemma":[0.0001134289,0.0002264457,0.00007813216,0.0002588184,0.000644832,0.00008262166,0.0001102328,0.0002517595,0.00001785148],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003082007,"about_ca_system_score_gemma":0.00008946257,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008822974,"about_ca_topic_score_gemma":0.000005714192,"domain_scores_codex":[0.9986256,0.00001096666,0.0003586251,0.000517928,0.0002102576,0.0002765844],"domain_scores_gemma":[0.9987281,0.0001290831,0.0002345504,0.0005363465,0.0002121251,0.0001598009],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001314234,0.00009636495,0.0005133987,0.0001419781,0.00008817974,0.000001331614,0.002220189,2.042194e-7,0.9939961,0.0002300104,0.00125895,0.001321803],"study_design_scores_gemma":[0.0009255848,0.00006144139,0.0004891792,0.00008311393,0.00008634714,0.00006644791,0.001002873,0.0001128431,0.9943792,0.001103584,0.001442639,0.0002468028],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9771296,0.0003984374,0.000007694031,0.001283485,0.00001491149,0.000171718,0.00004989794,0.00007448855,0.02086979],"genre_scores_gemma":[0.9973858,0.0001870966,0.00002955641,0.0002656284,0.000226561,0.00006129762,0.00008698168,0.00002972257,0.001727339],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02025624,"threshold_uncertainty_score":0.9234188,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01304826804298195,"score_gpt":0.269329192583861,"score_spread":0.2562809245408791,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}