{"id":"W2885348010","doi":"10.1002/cbic.201800305","title":"Quick Click: The DNA‐Templated Ligation of 3′‐<i>O</i>‐Propargyl‐ and 5′‐Azide‐Modified Strands Is as Rapid as and More Selective than Ligase","year":2018,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Grand Challenges Canada; Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","keywords":"DNA ligase; Azide; Chemistry; Oligonucleotide; Click chemistry; Propargyl; Combinatorial chemistry; Cycloaddition; DNA; Ligation; Chemical ligation; Bioproduction; Stereochemistry; Biochemistry; Organic chemistry; Biology; Peptide; Molecular biology; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008062565,0.0009673813,0.000492963,0.0003072201,0.0002095929,0.0006573582,0.0007228629,0.0006993829,0.006491082],"category_scores_gemma":[0.0006688958,0.0006489315,0.0003823491,0.0002872512,0.000457382,0.0007511037,0.0005429263,0.0007200128,0.002419385],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003103619,"about_ca_system_score_gemma":0.0003945023,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002016703,"about_ca_topic_score_gemma":0.000408049,"domain_scores_codex":[0.9990492,0.0001126488,0.00007439498,0.0003197234,0.0002969115,0.0001470563],"domain_scores_gemma":[0.999617,0.0001156498,0.00009891758,0.00004960652,0.00004564053,0.00007323857],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007447245,0.0000153733,0.0000585088,0.00007464772,0.00000637325,0.00007035312,0.00001673974,0.00003720443,0.9957916,0.0002240407,0.0003101193,0.003320687],"study_design_scores_gemma":[0.000008679808,0.00006487841,0.0002151953,0.000002013294,0.000003338844,0.0001300361,0.000003068027,0.0002556858,0.997074,0.00002207305,0.002214012,0.000007079228],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7633789,0.002956483,0.210135,0.0007186474,0.0007112555,0.0003595644,0.001162665,0.006238567,0.01433885],"genre_scores_gemma":[0.9134689,0.00129846,0.05713172,0.0003432671,0.0000890421,0.0003148308,0.001627852,0.0005419509,0.02518399],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006491082,"threshold_uncertainty_score":0.02171487,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01304826804298195,"score_gpt":0.269329192583861,"score_spread":0.2562809245408791,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}