{"id":"W2885552279","doi":"10.1128/jvi.01135-18","title":"HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site","year":2018,"lang":"en","type":"article","venue":"Journal of Virology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":32,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; Institute for Research in Immunology and Cancer; McGill University; Jewish General Hospital","funders":"Canadian Institutes of Health Research; Government of Canada","keywords":"Cas9; Biology; CRISPR; Subgenomic mRNA; Guide RNA; DNA; Reverse transcriptase; Genome editing; Primer (cosmetics); Virology; Human immunodeficiency virus (HIV); Genetics; RNA; Gene; Computational biology; Molecular biology; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003506951,0.0005262834,0.0004111557,0.000260239,0.0002889087,0.0008084434,0.0004957051,0.0006461383,0.0008718785],"category_scores_gemma":[0.0003014944,0.0002603996,0.0004430712,0.0001163401,0.0004542074,0.0005551369,0.0005101704,0.0008656015,0.0004015782],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005127337,"about_ca_system_score_gemma":0.0003786836,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005770489,"about_ca_topic_score_gemma":0.0008399059,"domain_scores_codex":[0.9996331,0.00003825552,0.00003592605,0.0001030486,0.0001331451,0.0000566729],"domain_scores_gemma":[0.9997784,0.00003977621,0.00006834431,0.00004947316,0.00003545189,0.00002863869],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000317992,0.00001407248,0.000490811,0.00004059852,0.00001241114,0.00006194988,0.00001531343,0.0003843074,0.9945146,0.0005687067,0.00003202227,0.003833474],"study_design_scores_gemma":[0.0000078884,0.0001145027,0.0009982215,0.000005149721,0.00001850934,0.0004972185,0.00001555703,0.002951693,0.9929403,0.0001960433,0.00224355,0.00001133276],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9188838,0.002631504,0.07253689,0.0003333729,0.00009239424,0.0001193383,0.0001410168,0.0006005758,0.004661134],"genre_scores_gemma":[0.9814371,0.0005198767,0.01556451,0.00007777803,0.000009489764,0.00002051496,0.0001225882,0.00003004046,0.002218128],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008718785,"threshold_uncertainty_score":0.003720164,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00881762885325819,"score_gpt":0.2849857487663345,"score_spread":0.2761681199130763,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}