{"id":"W2885552279","doi":"10.1128/jvi.01135-18","title":"HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site","year":2018,"lang":"en","type":"article","venue":"Journal of Virology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":32,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; Institute for Research in Immunology and Cancer; McGill University; Jewish General Hospital","funders":"Canadian Institutes of Health Research; Government of Canada","keywords":"Cas9; Biology; CRISPR; Subgenomic mRNA; Guide RNA; DNA; Reverse transcriptase; Genome editing; Primer (cosmetics); Virology; Human immunodeficiency virus (HIV); Genetics; RNA; Gene; Computational biology; Molecular biology; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000438979,0.0001406834,0.0001907999,0.00008434661,0.0001342754,0.00002331158,0.0002758417,0.0001279481,0.00003206541],"category_scores_gemma":[0.0003153943,0.0001051838,0.0001176198,0.00007699334,0.00006804271,0.000004747252,0.0001493735,0.0001559784,0.00004604251],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002303335,"about_ca_system_score_gemma":0.00003569439,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001291526,"about_ca_topic_score_gemma":0.00003781061,"domain_scores_codex":[0.9989096,0.00007279003,0.0003966204,0.0001822487,0.0001322371,0.0003065253],"domain_scores_gemma":[0.9993119,0.00003497203,0.0001790521,0.0002241833,0.0001445925,0.0001053614],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001080333,0.00002515794,0.0007823877,0.000003248864,0.00005293835,0.00001185806,0.0001208357,0.0005246667,0.9846737,0.00001232348,0.01306653,0.0006183241],"study_design_scores_gemma":[0.000467089,0.001170899,0.001689816,0.00001139369,0.00003995118,0.0001606138,0.00007089358,0.0001527063,0.8685511,0.00003856853,0.1275047,0.0001422482],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8949494,0.0004978937,0.1027983,0.0007749113,0.0006466464,0.0000841735,0.00001018197,0.000006338124,0.0002321743],"genre_scores_gemma":[0.9845722,0.00002525112,0.01287957,0.0006963258,0.001292391,0.000002590788,0.000008539136,0.00002838623,0.0004947498],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1161226,"threshold_uncertainty_score":0.428927,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00881762885325819,"score_gpt":0.2849857487663345,"score_spread":0.2761681199130763,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}