{"id":"W2886985642","doi":"10.3791/58149","title":"Quantification of Efferocytosis by Single-cell Fluorescence Microscopy","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Phagocytosis and Immune Regulation","field":"Immunology and Microbiology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Ontario Ministry of Research and Innovation; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Efferocytosis; Cell biology; Cell; Apoptosis; Flow cytometry; Biotinylation; Fluorescence microscope; Biology; Live cell imaging; Chemistry; Fluorescence; Macrophage; Molecular biology; Biochemistry; In vitro","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002271756,0.00103351,0.001308528,0.002228605,0.0008947268,0.001699498,0.001424451,0.001605801,0.003427417],"category_scores_gemma":[0.001833202,0.0005228728,0.0006434271,0.001496988,0.0009615293,0.001540288,0.001026392,0.002617437,0.001912679],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001310847,"about_ca_system_score_gemma":0.0008514281,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000858312,"about_ca_topic_score_gemma":0.00129437,"domain_scores_codex":[0.9977381,0.0003127051,0.0001913089,0.0005921854,0.0009327977,0.0002328812],"domain_scores_gemma":[0.9980444,0.0006600014,0.0002561573,0.0003325529,0.0006085265,0.00009836059],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000736155,0.00006654006,0.0004217772,0.0002399323,0.00001978838,0.00005146645,0.0001077947,0.000674737,0.9873096,0.001524111,0.0003818213,0.009128829],"study_design_scores_gemma":[0.00001343513,0.0001042501,0.002690082,0.00004614788,0.00002120922,0.0001251652,0.00007038042,0.01445805,0.9750261,0.0009461742,0.006466571,0.00003237068],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2293275,0.004346468,0.7462818,0.0003205183,0.0002962973,0.001183428,0.003054275,0.0032291,0.01196062],"genre_scores_gemma":[0.3790099,0.008506634,0.5950171,0.0002670778,0.00008687998,0.002630426,0.003154201,0.0006629961,0.01066475],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003427417,"threshold_uncertainty_score":0.01201433,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0325187212328693,"score_gpt":0.3898823025485862,"score_spread":0.3573635813157169,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}