{"id":"W2887088510","doi":"10.2144/btn-2018-0060","title":"Caution: Choice of Fixative can Influence the Visualization of the Location of a Transcription Factor in Mammalian Cells","year":2018,"lang":"en","type":"letter","venue":"BioTechniques","topic":"Poxvirus research and outbreaks","field":"Immunology and Microbiology","cited_by":3,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Fixative; Transcription factor; Biology; Computational biology; Cell biology; Visualization; Genetics; Chemistry; Computer science; Gene; Data mining; Cytoplasm","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002411857,0.0001541658,0.000273075,0.000172845,0.00005192725,0.000004456674,0.0004634438,0.0009167311,0.00005325833],"category_scores_gemma":[0.0001532434,0.0000943975,0.00008450036,0.0004294293,0.0007718744,0.00005520204,0.00005153623,0.0005966362,0.000002954666],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007372913,"about_ca_system_score_gemma":0.000166312,"about_ca_topic_candidate":true,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.006641725,"about_ca_topic_score_gemma":0.0007802421,"domain_scores_codex":[0.9984996,0.000582749,0.0004358135,0.0001857852,0.0001023897,0.0001936803],"domain_scores_gemma":[0.9986199,0.000120679,0.0004733877,0.0003862079,0.0003940547,0.000005790283],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004069261,0.00003210614,0.0004301539,0.0002958897,0.00003837752,3.241951e-7,0.0009992608,9.197636e-7,0.9500238,0.00006216486,0.04760236,0.0004739865],"study_design_scores_gemma":[0.000135415,0.000156041,0.01167258,0.0003983878,0.00001779817,0.000001278904,0.0000231151,0.000001420505,0.8997244,0.00008202452,0.08770763,0.00007991969],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7955614,0.005387301,0.004813113,0.1826711,0.001216843,0.006626327,0.003010924,0.0001956158,0.0005172793],"genre_scores_gemma":[0.9920862,0.0002135809,0.0000314784,0.007034474,0.00007483301,0.00004417473,0.0001539408,0.00001525294,0.0003460671],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1965247,"threshold_uncertainty_score":0.9999731,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02409072058086409,"score_gpt":0.2962978004121894,"score_spread":0.2722070798313254,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}