{"id":"W2887448192","doi":"10.1007/s10616-018-0231-7","title":"A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein–protein interactions mediated by G protein-coupled receptors","year":2018,"lang":"en","type":"article","venue":"Cytotechnology","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Canada Excellence Research Chairs, Government of Canada; Ministry of Education, Culture, Sports, Science and Technology","keywords":"HEK 293 cells; G protein-coupled receptor; Luciferase; Receptor; Transfection; Complementation; Molecular biology; Biology; Chemistry; Cell biology; Cell culture; Gene; Biochemistry; Genetics; Mutant","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003460296,0.0003204068,0.0003345417,0.0002139482,0.000390495,0.00006644642,0.0003199681,0.0004296819,0.0002938267],"category_scores_gemma":[0.0001372139,0.0003287914,0.00009984141,0.0003530014,0.0001370248,0.00001885304,0.00009951628,0.0002517275,0.00007610115],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000163519,"about_ca_system_score_gemma":0.00007874914,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001938313,"about_ca_topic_score_gemma":0.0001095487,"domain_scores_codex":[0.9978779,0.0001616415,0.0005386441,0.0006774011,0.0002117589,0.0005326798],"domain_scores_gemma":[0.9989825,0.000009905395,0.000161962,0.0004534849,0.0002466798,0.0001454877],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001949007,0.00009448511,0.000007378767,0.00003754756,0.00007620537,0.00000308182,0.0000778883,0.00002986177,0.9975822,0.0003735959,0.0002071005,0.001315743],"study_design_scores_gemma":[0.0005129399,0.0008805473,7.186019e-7,0.00009509515,0.00002104151,0.00001393411,0.0006795652,0.002395234,0.9904773,0.00008027508,0.004487853,0.0003554937],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8463695,0.00004923134,0.1509229,0.0003160242,0.0003003373,0.001737689,0.00009985458,0.0001792103,0.00002524175],"genre_scores_gemma":[0.9907337,0.00000245433,0.007383547,0.00005773928,0.0003965531,0.0006189245,0.0003017662,0.00007065863,0.000434692],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1443641,"threshold_uncertainty_score":0.9999164,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01658033125305719,"score_gpt":0.2675325185339612,"score_spread":0.250952187280904,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}