{"id":"W2888288916","doi":"10.21769/bioprotoc.2974","title":"Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor","year":2018,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Algal biology and biofuel production","field":"Energy","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Phaeodactylum tricornutum; Plasmid; Biology; CRISPR; Gene; Transformation (genetics); Cas9; Genetics; Algae; Botany","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000278638,0.0001935,0.0003092388,0.00006817222,0.0001500494,0.0000121975,0.0003078681,0.0003112118,0.0007071093],"category_scores_gemma":[0.00006547369,0.0001139507,0.0001142436,0.0002767075,0.0004175758,0.0001259287,0.0001171199,0.0001085674,0.00008317304],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000110636,"about_ca_system_score_gemma":0.0001294025,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.005459278,"about_ca_topic_score_gemma":0.0008818322,"domain_scores_codex":[0.9984805,0.0002311707,0.0005854819,0.0003381276,0.0001771187,0.0001876122],"domain_scores_gemma":[0.9986418,0.00005661055,0.0005262819,0.0004692995,0.0002634447,0.00004258656],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0105807,0.0002872654,0.006984596,0.0005824938,0.000185188,0.000003361273,0.000412767,0.000002742385,0.9649218,0.0008548834,0.00170599,0.01347821],"study_design_scores_gemma":[0.003034616,0.0009349178,0.0104375,0.0001453517,0.00005153932,0.00001307243,0.00010359,0.0001710956,0.9350399,0.0003419983,0.04952406,0.0002024035],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7586179,0.000002071641,0.0001053814,0.0001082086,0.001429705,0.2381406,0.00006878257,0.00009785313,0.001429443],"genre_scores_gemma":[0.8131685,1.310006e-7,0.0003100359,0.00003049642,0.001227757,0.1850714,0.00002327029,0.00001505213,0.0001533438],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05455056,"threshold_uncertainty_score":0.8252832,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01742992949425339,"score_gpt":0.2617495874896261,"score_spread":0.2443196579953727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}