{"id":"W2888288916","doi":"10.21769/bioprotoc.2974","title":"Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor","year":2018,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Algal biology and biofuel production","field":"Energy","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Phaeodactylum tricornutum; Plasmid; Biology; CRISPR; Gene; Transformation (genetics); Cas9; Genetics; Algae; Botany","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005874019,0.0009965042,0.0007250341,0.0006330913,0.0008913025,0.0007333359,0.0009813014,0.0007218083,0.008218745],"category_scores_gemma":[0.0004812819,0.0007584676,0.0005987415,0.0006143985,0.0003780632,0.0004496629,0.001112989,0.001730888,0.00468769],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006531224,"about_ca_system_score_gemma":0.0009280046,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001563002,"about_ca_topic_score_gemma":0.004253414,"domain_scores_codex":[0.9994938,0.00006168775,0.00007916507,0.0001434226,0.0001317777,0.00009014632],"domain_scores_gemma":[0.9997135,0.00004895347,0.00003638493,0.000101151,0.00004267774,0.00005739163],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007952469,0.00003297522,0.0001001437,0.0001236674,0.00000907767,0.00006985557,0.00003668262,0.00005489305,0.9950358,0.0004352508,0.0008981107,0.003124037],"study_design_scores_gemma":[0.00004031314,0.0001259,0.001525364,0.00002066437,0.00002997793,0.0002857944,0.00003248669,0.0007546229,0.9679036,0.000165256,0.02909388,0.00002218374],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5338138,0.003418039,0.3812066,0.001189193,0.001485632,0.007221425,0.02392605,0.007514152,0.0402251],"genre_scores_gemma":[0.5268969,0.003096579,0.2754104,0.0006037535,0.0001065382,0.004783386,0.0704636,0.001870714,0.1167681],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.008218745,"threshold_uncertainty_score":0.02749443,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01742992949425339,"score_gpt":0.2617495874896261,"score_spread":0.2443196579953727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}