{"id":"W2890351601","doi":"10.1007/978-1-4939-8730-6_1","title":"Transient Gene Expression in Suspension HEK293-EBNA1 Cells","year":2018,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Research Council Canada","funders":"","keywords":"HEK 293 cells; Transfection; Recombinant DNA; Polyethylenimine; Molecular biology; Cell culture; Gene; Embryo; Gene expression; Chemistry; Biology; Cell biology; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008210014,0.0002247159,0.000289596,0.0001837149,0.00003597455,0.0000100851,0.0003187026,0.0004022374,0.00006204149],"category_scores_gemma":[0.0001100114,0.0002068937,0.0001066628,0.0002001216,0.0001932387,0.000003251205,0.0001791023,0.000174285,0.0000234478],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002388522,"about_ca_system_score_gemma":0.00004973908,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004788335,"about_ca_topic_score_gemma":0.0001010366,"domain_scores_codex":[0.9975291,0.0009137691,0.0003855569,0.0006673218,0.00006897356,0.0004352982],"domain_scores_gemma":[0.999244,0.00002828578,0.00007288921,0.0005091981,0.00007596583,0.00006965053],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001540345,0.00007393443,0.0008371624,0.00000558672,0.00001010642,0.0000265457,0.00008819034,0.00004194789,0.9922813,0.00003361251,0.000168134,0.006279447],"study_design_scores_gemma":[0.0006022173,0.0006401628,0.0005253,0.00002590288,0.000005187213,0.00001379203,0.00004586373,0.0001098865,0.9896135,0.0005910739,0.007584673,0.0002424092],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5746168,0.0006132025,0.4235423,0.00005657742,0.0003541748,0.0001768156,0.000004493723,0.000007124333,0.0006285292],"genre_scores_gemma":[0.493161,0.0002643479,0.5053217,0.0007999461,0.0001578506,0.00004192645,0.00006505315,0.00003023131,0.0001579291],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.08177941,"threshold_uncertainty_score":0.843688,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02039937975275202,"score_gpt":0.3641160967270202,"score_spread":0.3437167169742681,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}