{"id":"W2890351601","doi":"10.1007/978-1-4939-8730-6_1","title":"Transient Gene Expression in Suspension HEK293-EBNA1 Cells","year":2018,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Research Council Canada","funders":"","keywords":"HEK 293 cells; Transfection; Recombinant DNA; Polyethylenimine; Molecular biology; Cell culture; Gene; Embryo; Gene expression; Chemistry; Biology; Cell biology; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009506955,0.000937802,0.001265747,0.0009049234,0.001011284,0.001318736,0.002162401,0.001228684,0.006269647],"category_scores_gemma":[0.0004194975,0.0008583578,0.0009760393,0.00141604,0.0006649671,0.000973733,0.0006811373,0.003732786,0.008093286],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007886032,"about_ca_system_score_gemma":0.0005636764,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001165024,"about_ca_topic_score_gemma":0.001554509,"domain_scores_codex":[0.9989153,0.0001224696,0.0001453421,0.0002949251,0.0003432168,0.0001787428],"domain_scores_gemma":[0.9995236,0.0001503439,0.00004552034,0.000129235,0.00009030304,0.00006099869],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009538825,0.00007285761,0.00007659582,0.0001094562,0.00001317548,0.00005370022,0.00007054346,0.0001098328,0.9955305,0.001029701,0.0006825069,0.002155676],"study_design_scores_gemma":[0.00003707582,0.00007146442,0.0005119672,0.00001055901,0.00002961489,0.0001119718,0.00002536862,0.001305129,0.9815899,0.0002092421,0.01608792,0.000009934251],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3830337,0.004963853,0.4719431,0.001621297,0.002057703,0.003730779,0.06437696,0.01151216,0.0567604],"genre_scores_gemma":[0.3786008,0.004645586,0.3848421,0.0009927677,0.0003726342,0.003985609,0.08635836,0.002759967,0.1374421],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006269647,"threshold_uncertainty_score":0.02097404,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02039937975275202,"score_gpt":0.3641160967270202,"score_spread":0.3437167169742681,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}