{"id":"W2890785410","doi":"10.1021/acs.molpharmaceut.8b00735","title":"Intracellular Delivery of Human Purine Nucleoside Phosphorylase by Engineered Diphtheria Toxin Rescues Function in Target Cells","year":2018,"lang":"en","type":"article","venue":"Molecular Pharmaceutics","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Institute of Infection and Immunity; Canadian Institutes of Health Research; Hospital for Sick Children; University of Toronto; Fondation Brain Canada","keywords":"Purine nucleoside phosphorylase; Diphtheria toxin; Intracellular; Cell biology; Induced pluripotent stem cell; Cytosol; Biology; Cytotoxic T cell; Biochemistry; Purine; Chemistry; Enzyme; Toxin; Embryonic stem cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001303686,0.0003478419,0.000285061,0.0001142529,0.00009136069,0.0003182012,0.000182594,0.0003131226,0.0006230334],"category_scores_gemma":[0.00009624612,0.0001305125,0.0001691246,0.0001098793,0.0001731706,0.0001572754,0.0002063123,0.0004552376,0.0003366131],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000345738,"about_ca_system_score_gemma":0.0002225377,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007715656,"about_ca_topic_score_gemma":0.0008055377,"domain_scores_codex":[0.9998955,0.00001366519,0.00001216802,0.00002889095,0.0000266293,0.00002323761],"domain_scores_gemma":[0.999938,0.0000166934,0.00001634785,0.00001112547,0.000006254077,0.00001164139],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002158952,0.000005917724,0.00002571489,0.00001318114,0.000001572279,0.00002623808,0.000008615454,0.00006658863,0.9993566,0.00005815568,0.0000110359,0.0004048702],"study_design_scores_gemma":[0.000005540465,0.00007900288,0.00031496,0.000001670102,0.000005023671,0.00007013485,0.000006211954,0.0005898239,0.998096,0.00001276469,0.0008174876,0.000001286201],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.986829,0.001029789,0.009721889,0.00008121691,0.00002645018,0.00004389261,0.0004438829,0.0001965881,0.001627356],"genre_scores_gemma":[0.9928411,0.0006171336,0.003716951,0.0000302082,0.000004240188,0.00003122293,0.0004189343,0.00003940738,0.002300859],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007715656,"threshold_uncertainty_score":0.002508521,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01482338701744599,"score_gpt":0.2957530408997894,"score_spread":0.2809296538823434,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}