{"id":"W2890785410","doi":"10.1021/acs.molpharmaceut.8b00735","title":"Intracellular Delivery of Human Purine Nucleoside Phosphorylase by Engineered Diphtheria Toxin Rescues Function in Target Cells","year":2018,"lang":"en","type":"article","venue":"Molecular Pharmaceutics","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Institute of Infection and Immunity; Canadian Institutes of Health Research; Hospital for Sick Children; University of Toronto; Fondation Brain Canada","keywords":"Purine nucleoside phosphorylase; Diphtheria toxin; Intracellular; Cell biology; Induced pluripotent stem cell; Cytosol; Biology; Cytotoxic T cell; Biochemistry; Purine; Chemistry; Enzyme; Toxin; Embryonic stem cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004596881,0.0002739699,0.0002431649,0.0001221752,0.00006996157,0.00002334168,0.0003596943,0.0002018065,0.0002635891],"category_scores_gemma":[0.00002486521,0.0003062206,0.0001164993,0.0002605121,0.0002129003,0.0000080096,0.0001596092,0.0002354377,0.00002784397],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000457937,"about_ca_system_score_gemma":0.00007566321,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003917939,"about_ca_topic_score_gemma":0.00003961488,"domain_scores_codex":[0.9981352,0.0002087673,0.0004004187,0.0004632435,0.0003136047,0.0004787686],"domain_scores_gemma":[0.9990095,0.00001008967,0.0001010384,0.0005408786,0.0001791507,0.0001593142],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001468746,0.0002690255,0.0001688022,0.00003856836,0.0001220178,0.00002605599,0.00005683364,0.0002984816,0.994335,0.00001076963,0.001821577,0.001384128],"study_design_scores_gemma":[0.001583794,0.002174748,0.0000376461,0.00001615114,0.00003135963,0.000002723314,0.00003141716,0.0008876711,0.9762704,0.00005492554,0.01861449,0.0002946414],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9927518,0.005011043,0.001331161,0.00006371802,0.0001600798,0.0003772371,0.00006232818,0.00002335416,0.0002193238],"genre_scores_gemma":[0.9980714,0.0004539184,0.0004422935,0.0002847645,0.0001445306,0.00002579487,0.0001980301,0.00008283279,0.0002963803],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01806456,"threshold_uncertainty_score":0.999939,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01482338701744599,"score_gpt":0.2957530408997894,"score_spread":0.2809296538823434,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}