{"id":"W2891006829","doi":"10.1038/s41596-018-0033-6","title":"Preparation of asymmetric phospholipid vesicles for use as cell membrane models","year":2018,"lang":"en","type":"article","venue":"Nature Protocols","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":166,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Windsor","funders":"","keywords":"Vesicle; Phospholipid; Liposome; Chemistry; Lipid bilayer; POPC; Phosphatidylcholine; Sphingomyelin; Biophysics; Membrane; Chromatography; Bilayer; Lipid bilayer phase behavior; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005554754,0.0005072868,0.0003829999,0.000414583,0.0005931098,0.0003630496,0.0005152863,0.0006119019,0.002691854],"category_scores_gemma":[0.000762792,0.0003217088,0.0003474723,0.0003937093,0.0001910221,0.0005921247,0.0005310311,0.001375943,0.001433777],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002834912,"about_ca_system_score_gemma":0.0002866638,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003337805,"about_ca_topic_score_gemma":0.0005555419,"domain_scores_codex":[0.9997838,0.00004617795,0.00002710228,0.00003981494,0.00006285965,0.00004034647],"domain_scores_gemma":[0.999799,0.00004587319,0.00002840608,0.00006054897,0.00004409104,0.00002221371],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008830654,0.00004359941,0.0001135323,0.00007512089,0.00001064244,0.00007483872,0.00004542645,0.0006138285,0.9925705,0.002575043,0.0002044765,0.003584728],"study_design_scores_gemma":[0.00003063543,0.00005570218,0.0001374473,0.000007886851,0.00001138826,0.0000520482,0.00001402005,0.002781209,0.9920487,0.0006242452,0.004230351,0.00000639078],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7384558,0.00129165,0.2439039,0.000724781,0.0002555596,0.001603409,0.00457557,0.0006280433,0.008561274],"genre_scores_gemma":[0.8532237,0.002234819,0.1315953,0.0001893311,0.00004217729,0.002297154,0.00434705,0.0002918254,0.005778653],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002691854,"threshold_uncertainty_score":0.009005189,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01891173485626842,"score_gpt":0.3420122461820115,"score_spread":0.3231005113257431,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}