{"id":"W2891155410","doi":"10.1016/j.jbiotec.2018.09.003","title":"Identification of an alternative translation initiation site in the sequence of the commonly used Glutathione S-Transferase tag","year":2018,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Montréal; Université Laval","funders":"Fonds de Recherche du Québec - Santé; Natural Sciences and Engineering Research Council of Canada; Fonds de recherche du Québec – Nature et technologies; Canadian Institutes of Health Research","keywords":"Fusion protein; Biology; Translation (biology); Computational biology; Protein biosynthesis; Molecular biology; Biochemistry; Genetics; Messenger RNA; Gene; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000365564,0.00006196778,0.0001056776,0.00009138167,0.00004693429,0.00000644291,0.0002782802,0.0001594468,0.000004246382],"category_scores_gemma":[0.00007464105,0.00003919086,0.00007617356,0.0001926855,0.0002385587,0.00001485742,0.00001680468,0.000131903,3.844074e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000905668,"about_ca_system_score_gemma":0.00005111186,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002918651,"about_ca_topic_score_gemma":0.0001825495,"domain_scores_codex":[0.9991181,0.0001738541,0.0004116064,0.00008963924,0.0001364303,0.00007031193],"domain_scores_gemma":[0.9991601,0.00001815988,0.0004316993,0.0002329654,0.0001435498,0.00001358579],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001116058,0.0001129232,0.001120683,0.000008721025,0.00001573057,0.000001112892,0.0005000683,0.000144195,0.9958207,0.000167715,0.00002588221,0.001970675],"study_design_scores_gemma":[0.0004742861,0.000803629,0.006806745,0.00002859971,0.00001937839,0.00003910579,0.0001844506,0.0002805525,0.9900625,0.001106177,0.0001525732,0.00004195175],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9950938,0.0001600802,0.003679712,0.000747834,0.0001523126,0.000115687,0.00003181401,0.000002078427,0.00001664806],"genre_scores_gemma":[0.999594,0.0001206807,0.00009658566,0.00009105404,0.00008022227,0.000002367593,0.000008623008,0.000004631835,0.000001877858],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005758137,"threshold_uncertainty_score":0.1598157,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02180312472212845,"score_gpt":0.3022222390323682,"score_spread":0.2804191143102397,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}