{"id":"W2891191674","doi":"10.21769/bioprotoc.3005","title":"Selective Isolation of Retroviruses from Extracellular Vesicles by Intact Virion Immunoprecipitation","year":2018,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Extracellular vesicles in disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"","keywords":"Immunoprecipitation; Viral envelope; Epitope; Glycoprotein; Nucleic acid; Murine leukemia virus; Biology; Virus; Cell biology; Cell culture; Virology; Vesicle; Virus-like particle; Chemistry; Retrovirus; Molecular biology; Biochemistry; Membrane; Antibody; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000501137,0.0008983613,0.0003429124,0.0005549275,0.0004583177,0.000959383,0.000843926,0.0005171027,0.002927524],"category_scores_gemma":[0.0005811615,0.0004427972,0.0005070219,0.0002956999,0.0003855445,0.0005695394,0.001181147,0.001526966,0.004362009],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003460629,"about_ca_system_score_gemma":0.0004105046,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000208008,"about_ca_topic_score_gemma":0.0005214359,"domain_scores_codex":[0.9995677,0.00006427489,0.00006931439,0.0001210504,0.0001116571,0.00006602946],"domain_scores_gemma":[0.9997854,0.00004240476,0.00003458613,0.00007264953,0.00003884298,0.00002623169],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005996952,0.00002367243,0.0001350861,0.0001319953,0.00001214593,0.0000671304,0.00004792372,0.00005001808,0.9901837,0.001021149,0.0004614608,0.007805821],"study_design_scores_gemma":[0.0000115578,0.00007296896,0.0009462677,0.00001637602,0.00002285673,0.0003829741,0.00001386324,0.0008914024,0.9810456,0.0003303016,0.01625713,0.000008727076],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3633176,0.00889525,0.5821139,0.0008679324,0.001289387,0.002055885,0.008995874,0.00295425,0.02950995],"genre_scores_gemma":[0.5770612,0.007096129,0.3338783,0.0005661905,0.0001872919,0.002434127,0.03061972,0.001107699,0.04704925],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002927524,"threshold_uncertainty_score":0.00979352,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01038960651679264,"score_gpt":0.2904802689290101,"score_spread":0.2800906624122174,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}