{"id":"W2897516664","doi":"10.1128/jb.00522-18","title":"Disrupted Synthesis of a Di- <i>N</i> -acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the <i>O</i> -Linked Protein Glycosylation System of Neisseria elongata subsp. <i>glycolytica</i>","year":2018,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Norges Forskningsråd; Wellcome Trust","keywords":"Neisseria; Glycosylation; Biology; Biochemistry; Glycan; Neisseria meningitidis; Mutant; Gene; Glycoconjugate; Glycoprotein; Biosynthesis; Glycosyltransferase; Microbiology; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00007752007,0.0002957723,0.0001114072,0.00009820929,0.00007918062,0.0002334646,0.0002214548,0.0001793328,0.0005007295],"category_scores_gemma":[0.0001064368,0.0001199575,0.0001821112,0.0000872067,0.0001897853,0.00008844931,0.0002020942,0.0003716544,0.0001884825],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001886773,"about_ca_system_score_gemma":0.000138418,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001156596,"about_ca_topic_score_gemma":0.001197274,"domain_scores_codex":[0.999902,0.00001494644,0.0000140538,0.0000200361,0.00002319067,0.00002571033],"domain_scores_gemma":[0.9998316,0.00002880003,0.00006145958,0.00002635445,0.00001130787,0.00004047566],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004439087,0.00001457633,0.0001415749,0.000004437074,0.000002318109,0.00002187187,0.000006540277,0.00001596133,0.9995496,0.00002313009,0.000004636853,0.0001710082],"study_design_scores_gemma":[0.000004398529,0.0001589059,0.005603046,0.000003005039,0.000009042538,0.0001475696,0.00003445263,0.000546108,0.9929351,0.00001994057,0.0005348395,0.000003623525],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9982388,0.00007906129,0.001105475,0.00002086812,0.000009033772,0.000009612238,0.0001714058,0.00002626009,0.0003394676],"genre_scores_gemma":[0.9980397,0.00007711758,0.000944827,0.00002367334,0.000002466969,0.000008147014,0.0002818712,0.00002212997,0.0005999469],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001156596,"threshold_uncertainty_score":0.002299666,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008283766448637785,"score_gpt":0.2483317282188567,"score_spread":0.2400479617702189,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}