{"id":"W2898119887","doi":"10.1002/cbic.201800538","title":"Post‐translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase","year":2018,"lang":"en","type":"review","venue":"ChemBioChem","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":55,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; University of Saskatchewan","funders":"Western Economic Diversification Canada; Canadian Cancer Society Research Institute","keywords":"Ubiquitin ligase; Posttranslational modification; DNA ligase; Chemistry; Protein evolution; Ubiquitin; Cell biology; Biochemistry; DNA; Biology; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003669959,0.000836606,0.0006999949,0.00169745,0.0002268237,0.0007382928,0.0006478226,0.0006779191,0.0023303],"category_scores_gemma":[0.0002852937,0.0002736155,0.0003353949,0.001634407,0.0002903908,0.001193193,0.0005630737,0.0009980501,0.002480477],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004467233,"about_ca_system_score_gemma":0.0005959941,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004824069,"about_ca_topic_score_gemma":0.0009908873,"domain_scores_codex":[0.9998784,0.00001111138,0.00001338949,0.00002632042,0.00005412634,0.00001673749],"domain_scores_gemma":[0.9999083,0.00002889237,0.00001905848,0.000004678591,0.00002717608,0.00001180606],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00003721337,0.00006160589,0.0001517811,0.01271116,0.0000594563,0.00027804,0.00004857562,0.0004452411,0.01805618,0.009748974,0.01974219,0.9386595],"study_design_scores_gemma":[0.000004794804,0.00005537626,0.0002315332,0.0005070652,0.00003068179,0.0007370054,0.00002399218,0.0001280057,0.006425113,0.0009462755,0.9908947,0.00001556291],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.00100171,0.9923244,0.001522047,0.0003348289,0.0003245821,0.00001634964,0.00005399424,0.0000458189,0.004376286],"genre_scores_gemma":[0.003630113,0.9902939,0.001498719,0.0001919083,0.0001119724,0.00002199598,0.00009738988,0.000007374142,0.004146729],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.0023303,"threshold_uncertainty_score":0.007795632,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03602222664543601,"score_gpt":0.3064874123678562,"score_spread":0.2704651857224202,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}