{"id":"W2898789738","doi":"10.1002/cpmb.79","title":"Polysome Profiling Analysis of mRNA and Associated Proteins Engaged in Translation","year":2018,"lang":"en","type":"article","venue":"Current Protocols in Molecular Biology","topic":"RNA modifications and cancer","field":"Biochemistry, Genetics and Molecular Biology","cited_by":86,"is_retracted":false,"has_abstract":true,"ca_institutions":"Beatrice Hunter Cancer Research Institute; Dalhousie University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Polysome; Messenger RNA; Translation (biology); Gene expression; Gene expression profiling; RNA; Biology; Ribosome profiling; Molecular biology; Protein biosynthesis; Sucrose gradient; Ultracentrifuge; Gene; Ribosome; Chemistry; Cell biology; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004222566,0.000779888,0.0005479648,0.002839353,0.0008191523,0.0005849796,0.0003540014,0.0003982546,0.00272677],"category_scores_gemma":[0.0003234276,0.0002433927,0.0004304838,0.001836599,0.0003455706,0.0004192153,0.0004753212,0.0009130699,0.001559369],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004352321,"about_ca_system_score_gemma":0.0004180414,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001242447,"about_ca_topic_score_gemma":0.003111841,"domain_scores_codex":[0.999575,0.00003374603,0.00002934843,0.0001225999,0.0001823987,0.00005700008],"domain_scores_gemma":[0.9997491,0.00004661389,0.0000418144,0.00002053778,0.0001028091,0.00003908567],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005661089,0.00001434679,0.0006358681,0.0001151356,0.00001426495,0.00005609592,0.00004991693,0.00004976112,0.9941607,0.0002545692,0.0002254685,0.004367181],"study_design_scores_gemma":[0.00001014406,0.0002218934,0.04114086,0.00004807082,0.0001110978,0.0004984082,0.0001145054,0.00354614,0.9371328,0.0005269579,0.01660505,0.00004412569],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7360885,0.009423566,0.2131691,0.0003832718,0.000381473,0.0007191812,0.02093269,0.002204652,0.01669772],"genre_scores_gemma":[0.6464266,0.01562274,0.2669398,0.0007286145,0.0002795604,0.00205205,0.03292858,0.001431721,0.03359025],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002839353,"threshold_uncertainty_score":0.009121954,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0433446029201536,"score_gpt":0.376270786771481,"score_spread":0.3329261838513274,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}