{"id":"W2899008101","doi":"10.1021/acssynbio.8b00339","title":"A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast","year":2018,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Plant tissue culture and regeneration","field":"Biochemistry, Genetics and Molecular Biology","cited_by":66,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Natural Sciences and Engineering Research Council of Canada; Concordia University","keywords":"Metabolic engineering; Synthetic biology; Gene; Computational biology; Biology; Genome engineering; CRISPR; Genetics; Saccharomyces cerevisiae; Transformation (genetics); DNA; Cas9","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002420974,0.0001790222,0.0002361829,0.00007295248,0.0000975085,0.00001994078,0.0001667165,0.0003229791,0.000006842316],"category_scores_gemma":[0.000150985,0.00013734,0.00006326263,0.00006646819,0.0001233907,0.000005358893,0.00004358358,0.00006303531,0.00003180303],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003584988,"about_ca_system_score_gemma":0.00004515978,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007663602,"about_ca_topic_score_gemma":0.0002868474,"domain_scores_codex":[0.9988524,0.0001213903,0.0002800824,0.0004264546,0.00004345075,0.0002762636],"domain_scores_gemma":[0.9994445,0.0000224364,0.000112455,0.0002739897,0.0000957514,0.000050796],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002028044,0.0000391538,0.0001680699,0.00002008466,0.00002356753,0.000001335808,0.0001216852,0.000001031444,0.9918121,0.0003588343,0.00008922938,0.007162157],"study_design_scores_gemma":[0.0007132245,0.000346231,0.0003172397,0.00008536147,0.00002798795,0.00006794384,0.00006731806,0.0006686778,0.9832905,0.00002614343,0.01419497,0.0001943997],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9820856,0.0004128225,0.01554312,0.0002762075,0.0007708642,0.0005200569,0.0001219601,0.00003131821,0.0002380918],"genre_scores_gemma":[0.9966888,0.00007843264,0.001383577,0.0000600296,0.0006054736,0.0001660268,0.0006334663,0.00002086672,0.0003633663],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0146032,"threshold_uncertainty_score":0.5600564,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01545675598342993,"score_gpt":0.2562646184835357,"score_spread":0.2408078625001057,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}