{"id":"W2900181173","doi":"10.1021/acs.bioconjchem.8b00649","title":"Visualization of the Delivery and Release of Small RNAs Using Genetic Code Expansion and Unnatural RNA-Binding Proteins","year":2018,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Foundation for Innovation","keywords":"RNA; Chemistry; Förster resonance energy transfer; microRNA; Small interfering RNA; Guide RNA; Cell biology; Computational biology; Gene silencing; RNA interference; Long non-coding RNA; RNA silencing; Biochemistry; Gene; Biology; Genome editing; Genome; Fluorescence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006637331,0.0001249707,0.0001224302,0.00001583432,0.00007268453,0.00001212198,0.0001219176,0.0001480206,0.000008067219],"category_scores_gemma":[0.00004625202,0.0001007078,0.00004446755,0.00006314533,0.0002961211,0.000004696489,0.0001670908,0.0000479413,3.300457e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008148784,"about_ca_system_score_gemma":0.00005942909,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004492433,"about_ca_topic_score_gemma":0.000007767198,"domain_scores_codex":[0.999303,0.00002426286,0.000218637,0.0002462577,0.00007969204,0.0001281597],"domain_scores_gemma":[0.9993885,0.00000512449,0.000173137,0.0002331687,0.0001530739,0.00004706536],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007400402,0.00001803286,0.002391798,0.0001251864,0.00002383825,7.523444e-7,0.00006193623,0.000006067821,0.9965169,0.000002772233,0.00002214066,0.0007565931],"study_design_scores_gemma":[0.000303037,0.0001069924,0.001820713,0.0001312851,0.00002918193,0.00002601349,0.00009612818,0.0005760323,0.9966612,0.00001630602,0.0001161002,0.0001169575],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998517,0.0008752366,0.0002838893,0.00001330742,0.00004883278,0.0001503743,0.000025697,0.000003926756,0.00008173387],"genre_scores_gemma":[0.9988844,0.0002845013,0.0005259963,0.00003131678,0.000108072,0.00000377598,0.0000183244,0.00001319707,0.0001304533],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006396355,"threshold_uncertainty_score":0.4106746,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01772935750989008,"score_gpt":0.2505054686548746,"score_spread":0.2327761111449845,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}