{"id":"W2900181173","doi":"10.1021/acs.bioconjchem.8b00649","title":"Visualization of the Delivery and Release of Small RNAs Using Genetic Code Expansion and Unnatural RNA-Binding Proteins","year":2018,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Foundation for Innovation","keywords":"RNA; Chemistry; Förster resonance energy transfer; microRNA; Small interfering RNA; Guide RNA; Cell biology; Computational biology; Gene silencing; RNA interference; Long non-coding RNA; RNA silencing; Biochemistry; Gene; Biology; Genome editing; Genome; Fluorescence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002155243,0.000309456,0.0001590999,0.0002215201,0.0001587948,0.0003104557,0.0002816329,0.0004110356,0.0005064821],"category_scores_gemma":[0.0001718246,0.0001512104,0.0001964471,0.0001579968,0.0003007826,0.0003192894,0.0002442361,0.0004985002,0.0002328846],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003870036,"about_ca_system_score_gemma":0.0002195507,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004898935,"about_ca_topic_score_gemma":0.0005213664,"domain_scores_codex":[0.9998391,0.00002621302,0.000008197587,0.00004962284,0.00005048978,0.00002628695],"domain_scores_gemma":[0.99989,0.00004076477,0.0000327866,0.0000106586,0.00001233462,0.00001354433],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001383736,0.000004394647,0.00002984697,0.00001203132,0.000001206844,0.00002621183,0.000008434764,0.00005421158,0.9989998,0.0001329663,0.00001341242,0.0007037113],"study_design_scores_gemma":[0.000002744653,0.00003021994,0.000285609,0.000001082819,0.000002099691,0.00005823558,0.000005790466,0.0009636623,0.9981457,0.00003429768,0.0004684748,0.000002097676],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9268449,0.001065571,0.06921842,0.0001835578,0.00003626462,0.00005047382,0.0001974391,0.0002976918,0.002105656],"genre_scores_gemma":[0.9304643,0.0012854,0.06437151,0.0001060113,0.00001497591,0.0001214133,0.0002421417,0.00009328399,0.003300925],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005064821,"threshold_uncertainty_score":0.002807856,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01772935750989008,"score_gpt":0.2505054686548746,"score_spread":0.2327761111449845,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}