{"id":"W2900259616","doi":"10.3791/57646","title":"Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Children's Hospital Research Institute of Manitoba","funders":"National Heart, Lung, and Blood Institute; Natural Sciences and Engineering Research Council of Canada; Studium Loire Valley-Institute for Advanced Studies; National Institutes of Health; HORIZON EUROPE Marie Sklodowska-Curie Actions; Children's Hospital Research Institute of Manitoba; Mitacs; Research Manitoba","keywords":"Prenylation; GTPase; Ras superfamily; Cell biology; GTP-binding protein regulators; GTP'; Biology; Rab; Ran; Small GTPase; G protein; GTPase-activating protein; Cytoskeleton; Signal transduction; Chemistry; Cell; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004782411,0.0001161652,0.0001728789,0.0001638308,0.0001202231,0.00002976587,0.00004564699,0.0001185746,0.00001677503],"category_scores_gemma":[0.0001189197,0.0001109428,0.00005870643,0.00008629124,0.00007277627,0.00002868607,0.00004044674,0.00006639301,4.11626e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003627838,"about_ca_system_score_gemma":0.00004209099,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001647162,"about_ca_topic_score_gemma":0.000002214024,"domain_scores_codex":[0.9989911,0.0001313471,0.000433918,0.0001485676,0.0001901428,0.0001049751],"domain_scores_gemma":[0.9989613,0.00001586467,0.0005842148,0.00009059489,0.0002781826,0.0000698509],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004326332,0.00006834137,0.000325294,0.0000212803,0.00007590294,0.000001101897,0.0001759161,0.00003541891,0.9960617,0.00001545742,0.000002443863,0.002784521],"study_design_scores_gemma":[0.001712079,0.0004240969,0.001177,0.00009169511,0.00004117271,0.00005807658,0.0001421138,0.003729694,0.9919927,0.00003422623,0.0004903887,0.0001067977],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9619067,0.001320639,0.03637738,0.00001251816,0.0002090364,0.0001425812,0.000002781713,0.000003955933,0.00002442833],"genre_scores_gemma":[0.9948797,0.0001545533,0.004634965,0.00002314271,0.0002566747,0.000002734406,0.0000118862,0.000014758,0.00002155609],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03297305,"threshold_uncertainty_score":0.4524118,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03060582810226575,"score_gpt":0.3677588807628857,"score_spread":0.33715305266062,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}