{"id":"W2900259616","doi":"10.3791/57646","title":"Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay","year":2018,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Children's Hospital Research Institute of Manitoba","funders":"National Heart, Lung, and Blood Institute; Natural Sciences and Engineering Research Council of Canada; Studium Loire Valley-Institute for Advanced Studies; National Institutes of Health; HORIZON EUROPE Marie Sklodowska-Curie Actions; Children's Hospital Research Institute of Manitoba; Mitacs; Research Manitoba","keywords":"Prenylation; GTPase; Ras superfamily; Cell biology; GTP-binding protein regulators; GTP'; Biology; Rab; Ran; Small GTPase; G protein; GTPase-activating protein; Cytoskeleton; Signal transduction; Chemistry; Cell; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001527176,0.00475559,0.001676418,0.00345062,0.0009669804,0.001125995,0.00196815,0.001809752,0.002971586],"category_scores_gemma":[0.001480308,0.0009375848,0.001510706,0.002217791,0.0009185773,0.001058691,0.001389581,0.003919011,0.004583481],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007478084,"about_ca_system_score_gemma":0.0007564754,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006174881,"about_ca_topic_score_gemma":0.001142414,"domain_scores_codex":[0.9973221,0.0005723826,0.0003475748,0.0006808491,0.0006522216,0.0004248963],"domain_scores_gemma":[0.9985265,0.0005246708,0.0001863826,0.000315207,0.0002880305,0.0001591352],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000822927,0.00007934423,0.00008679176,0.0001307584,0.0000174358,0.00004759231,0.00003096657,0.00004065587,0.9965076,0.0002776378,0.0001250499,0.002573767],"study_design_scores_gemma":[0.00002522999,0.000188598,0.001448145,0.00002403673,0.00004532226,0.0002833584,0.00002733091,0.001376537,0.9906481,0.000377759,0.00552204,0.00003345495],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1835393,0.0141239,0.7795151,0.0008125661,0.001164833,0.001781808,0.003944265,0.005093256,0.01002508],"genre_scores_gemma":[0.3348115,0.01500499,0.6127343,0.0007449035,0.0004351843,0.004409772,0.01534678,0.0007697486,0.01574291],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00475559,"threshold_uncertainty_score":0.009940982,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03060582810226575,"score_gpt":0.3677588807628857,"score_spread":0.33715305266062,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}