{"id":"W2901371681","doi":"10.1016/j.mimet.2018.11.017","title":"Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices","year":2018,"lang":"en","type":"article","venue":"Journal of Microbiological Methods","topic":"Parasitic Infections and Diagnostics","field":"Immunology and Microbiology","cited_by":23,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research; ArcticNet; Natural Sciences and Engineering Research Council of Canada; University of Arizona; National Science Foundation","keywords":"Cryptosporidium parvum; Toxoplasma gondii; Cryptosporidium; Biology; Nucleic acid; Apicomplexa; DNA extraction; DNA; Parasite hosting; Microbiology; Extraction (chemistry); Polymerase chain reaction; Virology; Gene; Chromatography; Biochemistry; Chemistry; Protozoal disease; Genetics; Feces","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001839622,0.000479289,0.0004266538,0.0005135833,0.0004378258,0.0004690543,0.0005472349,0.0005812952,0.001295143],"category_scores_gemma":[0.003762817,0.000370857,0.0004206638,0.0004618924,0.0003327793,0.000589554,0.0002651686,0.0004806686,0.0003426322],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003453293,"about_ca_system_score_gemma":0.0004969505,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001506993,"about_ca_topic_score_gemma":0.004004856,"domain_scores_codex":[0.9991657,0.0001720506,0.0001111637,0.0001613543,0.000287737,0.0001020481],"domain_scores_gemma":[0.9971045,0.00155215,0.000217142,0.0001409557,0.000893421,0.00009181866],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001712663,0.0002117713,0.006521985,0.000260957,0.00005090763,0.00009693932,0.0003196422,0.0009688096,0.9699668,0.0001053589,0.0002144207,0.01956987],"study_design_scores_gemma":[0.00005025909,0.003406091,0.03774062,0.00004586287,0.0001910135,0.0003703758,0.0002490903,0.004271744,0.9511287,0.0001549108,0.002348132,0.00004320894],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.981303,0.002937743,0.01373554,0.0001226087,0.0001177097,0.0001766992,0.0004472396,0.0001192588,0.001040175],"genre_scores_gemma":[0.9564699,0.002986109,0.0364746,0.0001491259,0.00004298436,0.0003155079,0.001751546,0.000104062,0.001706246],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001839622,"threshold_uncertainty_score":0.009728968,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02263280218684114,"score_gpt":0.3713522723273963,"score_spread":0.3487194701405551,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}