{"id":"W2901491142","doi":"10.1042/bcj20180763","title":"Structural enzymology reveals the molecular basis of substrate regiospecificity and processivity of an exemplar bacterial glycoside hydrolase family 74 <i>endo</i>-xyloglucanase","year":2018,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; University of Toronto; Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"","keywords":"Processivity; Glycoside hydrolase; Hydrolase; Chemistry; Stereochemistry; Biochemistry; Active site; Enzyme; Polymerase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003097551,0.0001622298,0.0002164976,0.00003863617,0.00010765,0.00002758539,0.0002339311,0.0001740998,0.00002951861],"category_scores_gemma":[0.0001564245,0.0001217774,0.00007739147,0.00009428721,0.000488591,0.00001623698,0.00007477147,0.0001610491,5.222651e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009922494,"about_ca_system_score_gemma":0.0001021765,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001541306,"about_ca_topic_score_gemma":0.000005427652,"domain_scores_codex":[0.9987469,0.0001729285,0.0004116353,0.0002943553,0.0001674779,0.0002066501],"domain_scores_gemma":[0.9988714,0.00001096341,0.0003844221,0.0002952103,0.0002994256,0.0001385341],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005471515,0.00004604077,0.0009075964,0.00002179352,0.00004014851,0.000004950331,0.00003672823,0.00000118227,0.9974935,0.00005655624,0.0004533511,0.0003910229],"study_design_scores_gemma":[0.0005640773,0.000391242,0.007957648,0.00001627434,0.0000345214,0.0003734044,0.00004403817,0.00002100275,0.9896311,0.000221555,0.0006149811,0.0001301416],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987103,0.0003990941,0.0002057752,0.0002855571,0.0001964756,0.000110733,0.00004926948,0.00000485435,0.00003795145],"genre_scores_gemma":[0.9983556,0.0001160888,0.0005051282,0.0002331701,0.0006859929,0.000003130217,0.00006725804,0.00001535759,0.00001825617],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007862364,"threshold_uncertainty_score":0.4965939,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01190213024285142,"score_gpt":0.2456385628730753,"score_spread":0.2337364326302239,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}