{"id":"W2902330969","doi":"10.1371/journal.pone.0208075","title":"Peak emission wavelength and fluorescence lifetime are coupled in far-red, GFP-like fluorescent proteins","year":2018,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Sunnybrook Health Science Centre","funders":"Eli Lilly and Company","keywords":"Fluorescence; Green fluorescent protein; Förster resonance energy transfer; Biophysics; Bimolecular fluorescence complementation; Aequorea victoria; Fluorescence in the life sciences; Yellow fluorescent protein; Fluorescence correlation spectroscopy; Fluorescence microscope; Fluorescence-lifetime imaging microscopy; Fluorescent protein; Biology; Chemistry; Biochemistry; Optics; Gene; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001895773,0.00028901,0.0003031057,0.00008869354,0.0001241591,0.00003300152,0.0003063796,0.0002616317,0.00003443582],"category_scores_gemma":[0.0002472784,0.0002824212,0.00004202987,0.0001650585,0.0003145766,0.00001373457,0.0003169491,0.0002345512,0.00001528805],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004104989,"about_ca_system_score_gemma":0.00006452383,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003123494,"about_ca_topic_score_gemma":0.00005173661,"domain_scores_codex":[0.9981679,0.00006384686,0.0003204728,0.0006977401,0.0002759883,0.0004740426],"domain_scores_gemma":[0.9989059,0.000009645962,0.0001430022,0.0005810467,0.0001911318,0.0001692539],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000218419,0.000576597,0.0109255,0.00009000721,0.00003645268,0.000007370043,0.00007113506,2.344831e-7,0.9862025,0.00001036481,0.0004116851,0.001449787],"study_design_scores_gemma":[0.0006050402,0.0006004619,0.006723296,0.0004776031,0.00002854286,0.000005437174,0.00003877426,0.001475549,0.9883685,0.00007649564,0.001241479,0.0003588786],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9949221,0.00086988,0.002295006,0.0004200984,0.00004305561,0.001170447,0.00003086631,0.0001116096,0.0001369592],"genre_scores_gemma":[0.927913,0.001044628,0.06967055,0.0003286239,0.0002862957,0.0001325407,0.00006584032,0.00006533987,0.0004932333],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06737554,"threshold_uncertainty_score":0.9999628,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01529033950698896,"score_gpt":0.245737445519463,"score_spread":0.230447106012474,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}