{"id":"W2902330969","doi":"10.1371/journal.pone.0208075","title":"Peak emission wavelength and fluorescence lifetime are coupled in far-red, GFP-like fluorescent proteins","year":2018,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Sunnybrook Health Science Centre","funders":"Eli Lilly and Company","keywords":"Fluorescence; Green fluorescent protein; Förster resonance energy transfer; Biophysics; Bimolecular fluorescence complementation; Aequorea victoria; Fluorescence in the life sciences; Yellow fluorescent protein; Fluorescence correlation spectroscopy; Fluorescence microscope; Fluorescence-lifetime imaging microscopy; Fluorescent protein; Biology; Chemistry; Biochemistry; Optics; Gene; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009023615,0.0004914503,0.0005631671,0.0006510572,0.0003880226,0.0008313713,0.0007280255,0.001009896,0.001432601],"category_scores_gemma":[0.001241094,0.0004969978,0.000511956,0.001225434,0.0008613163,0.001374301,0.0006146146,0.001417658,0.001046836],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00131949,"about_ca_system_score_gemma":0.0006915231,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007525847,"about_ca_topic_score_gemma":0.0006374916,"domain_scores_codex":[0.9991659,0.0001064106,0.00003553562,0.000362125,0.0002523788,0.0000777387],"domain_scores_gemma":[0.9990968,0.0002800684,0.00025874,0.00008372592,0.0002052411,0.00007555377],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008523837,0.00002315258,0.0006815003,0.0001904375,0.00001313297,0.00008330783,0.00005202469,0.0006917131,0.9874737,0.002955558,0.0001515702,0.00759858],"study_design_scores_gemma":[0.00000583708,0.00005745509,0.004253822,0.00001786992,0.00002312865,0.0005712141,0.00003810137,0.004604937,0.9844723,0.001571988,0.00435343,0.00002992385],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6243526,0.01062164,0.3490263,0.001349358,0.0002084212,0.00009629207,0.001582923,0.001985256,0.01077727],"genre_scores_gemma":[0.8617126,0.004452605,0.125347,0.0005064628,0.00004355197,0.0001970492,0.0009274483,0.0003110992,0.006502144],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001432601,"threshold_uncertainty_score":0.009573579,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01529033950698896,"score_gpt":0.245737445519463,"score_spread":0.230447106012474,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}