{"id":"W2902340796","doi":"10.1039/c8ra08515c","title":"Protein-induced fluorescence enhancement for a simple and universal detection of protein/small molecule interactions","year":2018,"lang":"en","type":"article","venue":"RSC Advances","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Kootenay Association for Science & Technology","funders":"Ministry of Science, ICT and Future Planning; National Research Foundation","keywords":"Fluorescence; Biotin; Streptavidin; Chemistry; Biophysics; Small molecule; Folate receptor; Target protein; Biotinylation; Protein–protein interaction; Biological system; Biochemistry; Biology; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006599082,0.0008062553,0.0004772584,0.0003908003,0.000243797,0.0002924443,0.0005494477,0.00104378,0.001064897],"category_scores_gemma":[0.0006482942,0.0003195342,0.0003140108,0.0002604885,0.0004606757,0.0004616035,0.000488758,0.00104898,0.0007393939],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000451199,"about_ca_system_score_gemma":0.0002956757,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000559033,"about_ca_topic_score_gemma":0.001019031,"domain_scores_codex":[0.9994742,0.0001349108,0.00002447915,0.0001362443,0.0001684916,0.00006175505],"domain_scores_gemma":[0.9996938,0.0001356986,0.00005544274,0.00003494313,0.00005286213,0.00002725932],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000188736,0.00001228189,0.0000815563,0.00009187986,0.000004561295,0.0000391026,0.00001985594,0.00008911367,0.9958375,0.000367659,0.00008985648,0.003347875],"study_design_scores_gemma":[0.000003529586,0.00006666589,0.000265184,0.000004179839,0.000005809522,0.0001686133,0.000008667776,0.001921503,0.9947144,0.00005873584,0.002775335,0.000007370263],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4145319,0.01247101,0.5630067,0.00070501,0.0002398935,0.0003831944,0.0003494186,0.0014794,0.006833531],"genre_scores_gemma":[0.7272629,0.003713971,0.263234,0.0002768491,0.00004841958,0.0002529347,0.0002679379,0.00006617441,0.004876855],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001064897,"threshold_uncertainty_score":0.00356245,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01078044296867743,"score_gpt":0.264188504213156,"score_spread":0.2534080612444786,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}