{"id":"W2902400937","doi":"10.1186/s12896-018-0485-3","title":"High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension","year":2018,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"MicroRNA in disease regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Université du Québec à Montréal; Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Dicer; Biology; Transfection; HEK 293 cells; Molecular biology; Recombinant DNA; Ribonuclease; Cell culture; Cell biology; Biochemistry; Gene; RNA; Genetics; Small interfering RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001877158,0.0008298157,0.00173152,0.000678036,0.0006230417,0.00110062,0.001147591,0.001261885,0.002970825],"category_scores_gemma":[0.001011649,0.0005381469,0.0006709229,0.001131343,0.000520424,0.0006288313,0.0008400977,0.001874609,0.005358343],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000701785,"about_ca_system_score_gemma":0.0004237981,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005418755,"about_ca_topic_score_gemma":0.0009015601,"domain_scores_codex":[0.9984865,0.000231615,0.0002372603,0.0003502067,0.0005423592,0.0001519952],"domain_scores_gemma":[0.999508,0.0001154282,0.00005973846,0.0001349941,0.0001332753,0.00004856207],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006268112,0.00006417864,0.0001106721,0.0001737473,0.00002081542,0.0001130855,0.0000833276,0.0003275545,0.9948938,0.000588947,0.0008510798,0.002709983],"study_design_scores_gemma":[0.00004882377,0.0001663984,0.0005624394,0.00002795544,0.00004021795,0.0003259151,0.0000278311,0.003725469,0.9746861,0.0001733153,0.02018803,0.00002744664],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4309816,0.009934759,0.50413,0.001262103,0.001212469,0.003803828,0.02468185,0.004714309,0.01927917],"genre_scores_gemma":[0.5561149,0.006528358,0.3612203,0.0008246736,0.0001953409,0.003025716,0.04487913,0.001346722,0.02586479],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002970825,"threshold_uncertainty_score":0.009938359,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0105448414707651,"score_gpt":0.2426989518348442,"score_spread":0.2321541103640791,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}