{"id":"W2902400937","doi":"10.1186/s12896-018-0485-3","title":"High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension","year":2018,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"MicroRNA in disease regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Université du Québec à Montréal; Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Dicer; Biology; Transfection; HEK 293 cells; Molecular biology; Recombinant DNA; Ribonuclease; Cell culture; Cell biology; Biochemistry; Gene; RNA; Genetics; Small interfering RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001650113,0.0001337983,0.0002045565,0.0001840747,0.00005292173,0.000002715137,0.0001504774,0.0006008246,0.00004263258],"category_scores_gemma":[0.00004403053,0.0001399591,0.00006383844,0.0002206509,0.0004016533,0.000005792547,0.00005813905,0.0001053245,0.000003709749],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002489927,"about_ca_system_score_gemma":0.00002511872,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002974528,"about_ca_topic_score_gemma":0.0009613318,"domain_scores_codex":[0.9989375,0.00005626714,0.0003518169,0.0003895214,0.00009002091,0.0001748779],"domain_scores_gemma":[0.9992731,0.000004076805,0.0001666723,0.0004424941,0.00009065497,0.00002298106],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006305355,0.0001630841,0.0007922782,0.00006848153,0.00001510857,2.501843e-7,0.00001983372,0.000007276401,0.9968808,0.0004090086,0.001081063,0.000499778],"study_design_scores_gemma":[0.0004214732,0.0007932529,0.004698704,0.00003832152,0.00001934189,0.000003942334,0.00003132055,0.000006534671,0.9931034,0.0003107698,0.0004467607,0.0001261902],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976022,0.0001851676,0.001434341,0.00013847,0.0002095906,0.0003344548,0.000010528,0.00002868507,0.00005658022],"genre_scores_gemma":[0.9991076,0.00004256033,0.0004805635,0.00001047361,0.0001138978,0.00001269656,0.00009911438,0.0000193235,0.0001137495],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003906426,"threshold_uncertainty_score":0.5707366,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0105448414707651,"score_gpt":0.2426989518348442,"score_spread":0.2321541103640791,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}