{"id":"W2903560436","doi":"10.1002/jcp.27890","title":"Serum‐reduced media impacts on cell viability and protein expression in human lung epithelial cells","year":2018,"lang":"en","type":"article","venue":"Journal of Cellular Physiology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":93,"is_retracted":false,"has_abstract":true,"ca_institutions":"Children's Hospital Research Institute of Manitoba; University of Manitoba; Research Manitoba","funders":"Canadian Institutes of Health Research","keywords":"Transfection; Gene knockdown; Viability assay; A549 cell; Small interfering RNA; Fetal bovine serum; Intracellular; Molecular biology; Cell biology; Biology; Cell culture; Cell; Messenger RNA; Heat shock protein; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003471825,0.0001672904,0.0002559607,0.00008388182,0.0000763628,0.000008254057,0.0002225351,0.0002712219,0.00002994215],"category_scores_gemma":[0.0000444264,0.000134146,0.0000878936,0.0000745254,0.00022777,0.00000497558,0.0001077333,0.0002296153,0.000002666339],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001833598,"about_ca_system_score_gemma":0.00005539347,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005759935,"about_ca_topic_score_gemma":0.000005322129,"domain_scores_codex":[0.9987875,0.0002216938,0.0003796158,0.0002871615,0.00008823068,0.0002358417],"domain_scores_gemma":[0.9991435,0.00001716324,0.0002959845,0.0003154482,0.0001205221,0.0001073841],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001550221,0.0001654041,0.0001044703,0.00001897367,0.00000988737,0.000004346556,0.00004636155,0.000004508761,0.9988735,0.00005372542,0.0004243875,0.0001394459],"study_design_scores_gemma":[0.0006038918,0.001085149,0.003745836,0.0000277929,0.00000934022,0.000008539926,0.00001698574,0.00001601526,0.9911022,0.0009081743,0.002341961,0.000134141],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981473,0.000152852,0.001018505,0.00007739859,0.00008269001,0.0002574034,0.00001380216,0.000005144187,0.0002448993],"genre_scores_gemma":[0.9982437,0.00004427459,0.001043968,0.0001084247,0.0004567957,0.00001318363,0.00002262813,0.00001745141,0.00004950963],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007771294,"threshold_uncertainty_score":0.5470313,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006135975341065818,"score_gpt":0.2567341543080959,"score_spread":0.2505981789670301,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}