{"id":"W2903560436","doi":"10.1002/jcp.27890","title":"Serum‐reduced media impacts on cell viability and protein expression in human lung epithelial cells","year":2018,"lang":"en","type":"article","venue":"Journal of Cellular Physiology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":93,"is_retracted":false,"has_abstract":true,"ca_institutions":"Children's Hospital Research Institute of Manitoba; University of Manitoba; Research Manitoba","funders":"Canadian Institutes of Health Research","keywords":"Transfection; Gene knockdown; Viability assay; A549 cell; Small interfering RNA; Fetal bovine serum; Intracellular; Molecular biology; Cell biology; Biology; Cell culture; Cell; Messenger RNA; Heat shock protein; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004105264,0.0002992227,0.0004166188,0.0002667984,0.0004459003,0.0005958223,0.0002088887,0.0002810626,0.00129253],"category_scores_gemma":[0.0003269862,0.0001299869,0.0003536649,0.0003959572,0.0002111778,0.0002463249,0.000274651,0.0004786163,0.0004711696],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003494676,"about_ca_system_score_gemma":0.0003578953,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002536836,"about_ca_topic_score_gemma":0.00349371,"domain_scores_codex":[0.999355,0.0001333339,0.0001009971,0.00009533164,0.0002473792,0.00006783572],"domain_scores_gemma":[0.9997372,0.00007382559,0.00003407057,0.00003043895,0.00010332,0.00002105969],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002441338,0.00007162184,0.0005038722,0.0001620405,0.000014482,0.00008468026,0.00009353863,0.00007431998,0.9955742,0.00009118291,0.0002186939,0.002867205],"study_design_scores_gemma":[0.000009917597,0.0004146326,0.004623952,0.00001333453,0.00003282812,0.0001408756,0.0001026458,0.0005816327,0.9900018,0.00004058915,0.004028212,0.000009617893],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9744186,0.01210527,0.004852306,0.0003469757,0.0002071544,0.00007356348,0.002048924,0.0001964512,0.005750703],"genre_scores_gemma":[0.9812886,0.004845852,0.005018823,0.0002320851,0.00002011696,0.0001544319,0.003212966,0.00005285965,0.005174316],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002536836,"threshold_uncertainty_score":0.005044162,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006135975341065818,"score_gpt":0.2567341543080959,"score_spread":0.2505981789670301,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}