{"id":"W2905157294","doi":"10.1042/bcj20180787","title":"Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity","year":2018,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Lipid metabolism and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"","keywords":"Biochemistry; Amino acid; Arabidopsis; Biology; Enzyme; Arabidopsis thaliana; Metabolic engineering; Fatty acid synthesis; Fatty acid; Acyl-CoA; Biosynthesis; Metabolic pathway; Residue (chemistry); Mutant; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003283147,0.0007454743,0.0004296817,0.000468535,0.0001522393,0.0004313827,0.0005379928,0.0003693656,0.0008522138],"category_scores_gemma":[0.0002873229,0.0002452717,0.0004951497,0.0004971782,0.0003109382,0.0001748195,0.0004482455,0.000670546,0.0004649245],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005603667,"about_ca_system_score_gemma":0.0003216453,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007851319,"about_ca_topic_score_gemma":0.0009305319,"domain_scores_codex":[0.9996711,0.00006250366,0.00006071261,0.00006031682,0.00009223533,0.00005317685],"domain_scores_gemma":[0.9996243,0.00007589682,0.0001339182,0.00004594442,0.00003838206,0.00008158297],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005869746,0.0000262672,0.0001084803,0.00002101829,0.000007086307,0.00007485293,0.00001121179,0.0001619731,0.99867,0.00008070816,0.00001616243,0.0007635515],"study_design_scores_gemma":[0.0000383542,0.0001835873,0.001915617,0.000005575952,0.00003914795,0.0002741677,0.00003480745,0.002727017,0.992364,0.00005267666,0.002350958,0.0000141598],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9866452,0.0002530389,0.01101346,0.0001074631,0.00006831846,0.0001109264,0.0007284833,0.0002674505,0.0008056653],"genre_scores_gemma":[0.9846416,0.0002535089,0.01100951,0.00004470666,0.00002215645,0.00006297091,0.001540969,0.0001703475,0.002254234],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008522138,"threshold_uncertainty_score":0.004065812,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006050244709708312,"score_gpt":0.2151229423482615,"score_spread":0.2090726976385532,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}